Biodesign of a skeletal muscle flap as a model for cardiac assistance
| Autor: | Irene Hernandez, Peter I. Lelkes, Matthew D. Silverman, Victor V. Nikolaychik, Valeri S. Chekanov |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Free Radicals Proline medicine.medical_treatment Muscle Fibers Skeletal Biomedical Engineering Medicine (miscellaneous) Neovascularization Physiologic Bioengineering Transplantation Autologous Fibrin Surgical Flaps Biomaterials Neovascularization Thrombin medicine Animals Cardiomyoplasty Muscle Skeletal Sheep biology Latissimus dorsi muscle Graft Survival Skeletal muscle Fibrinogen General Medicine Free Radical Scavengers Free radical scavenger Surgery Capillaries Transplantation Microscopy Electron medicine.anatomical_structure Delayed-Action Preparations Reperfusion Injury biology.protein Tissue Adhesives medicine.symptom medicine.drug |
| Zdroj: | Artificial organs. 24(2) |
| ISSN: | 0160-564X |
| Popis: | In using autologous muscles for cardiac assistance, it is crucial to reduce ischemia-reperfusion injury in the surgically traumatized skeletal muscle. In adult sheep, we developed a simple model of surgically designed 2 latissimus dorsi muscle leaflets by modifying the vascular supply to these leaflets. Three pockets with graded injury were established, and muscle morphology and vascular remodeling were monitored in 3 experimental groups: muscle leaflets without any treatment (Group 1, n = 6) that served as controls; muscle leaflets integrated with a fibrin interlayer (Group 2, n = 6); and leaflets integrated with fibrin and entrapped pyrrolostatin (Group 3, n = 6). We applied the fibrinogen and thrombin solutions, which polymerize to form a three-dimensional meshwork joining the tissues, creating a provisional matrix for angiogenesis, and acting as a delivery depot for agents aimed at minimizing ischemia-reperfusion lesion formation. After 2 months, the muscle leaflets biointegrated with the fibrin interface showed none of the signs of necrosis or ischemia-reperfusion lesions seen in the controls. Although no angiogenic factors were incorporated, the fibrin interlayer rapidly ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|