Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanae
| Autor: | Takafumi Fujimoto, Laura Satiko Okada Nakaghi, Nivaldo Ferreira do Nascimento, José Augusto Senhorini, Nycolas Levy Pereira, Matheus Pereira dos Santos, George Shigueki Yasui |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Natl Ctr Res & Conservat Continental Fish, Hokkaido Univ |
| Rok vydání: | 2018 |
| Předmět: |
Morphology
Male 0301 basic medicine Embryo Nonmammalian Gametes medicine.medical_treatment media_common.quotation_subject Fertilization in Vitro Biology Polyploidy Andrology 03 medical and health sciences Human fertilization medicine Animals media_common Ploidies In vitro fertilisation Characidae Hatching Reproduction Embryo 04 agricultural and veterinary sciences Cell Biology Oocyte 030104 developmental biology medicine.anatomical_structure Larva Embryogenesis Oocytes 040102 fisheries 0401 agriculture forestry and fisheries Gamete Female Ploidy Developmental Biology |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199417000739 |
| Popis: | Made available in DSpace on 2018-11-26T17:45:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) In fish, many factors can affect reproduction during in vitro fertilization, therefore determination of the factors that affect affecting gamete quality is needed. However, few studies have focused on gamete quality and the ploidy status. This study was conducted to elucidate whether oocyte storage can affect ploidy status, survival, and embryo viability in the characid species Astyanax altiparanae. Oocytes were stored in Dulbecco's phosphate-buffered saline (PBS) at 26 degrees C, then aliquots were fertilized immediately after extrusion (control) and also after 60, 120, 180, and 240 min of storage. Fertilization and hatching rates were measured, and the developmental stages were analyzed at each stage before describing the main abnormalities. Ploidy status was analyzed by flow cytometry and blood smear. In the control group, 100% of the samples were diploid. After treatment for 60 min, 95.56 +/- 4.44% samples were diploid and 4.44 +/- 4.44% were triploid. After 120 min, 94.44 +/- 9.62% of the samples was diploid and 5.56 +/- 5.56% were triploid; 100% of the samples were diploid after 180 min and, after 240 min, there was no survival. In other treatments, the highest percentage of hatching was after 60 min (88.93 +/- 5.15%; P = 0.015), and treatment with 180 min storage resulted in the highest percentage of abnormal larvae (95.76 +/- 12.67%; P = 0.012). These results show that oocyte storage can affect ploidy status and may be an interesting parameter for analysis in studies on chromosome set manipulation and micromanipulation. Sao Paulo State Univ, Aquaculture Ctr, Jaboticabal, Brazil Natl Ctr Res & Conservat Continental Fish, Chico Mendes Inst Biodivers Conservat, Pirassununga, Brazil Hokkaido Univ, Fac Fisheries Sci, Hakodate, Hokkaido, Japan Sao Paulo State Univ, Aquaculture Ctr, Jaboticabal, Brazil FAPESP: 2010/17429-1 |
| Databáze: | OpenAIRE |
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