A rapid and simple protocol to prepare a living biocomposite that mimics electroactive biofilms
| Autor: | Manuel Dossot, Stéphane Pinck, Mathieu Etienne, Frédéric Jorand |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Shewanella
Nanotube Time Factors Cytochrome Bioelectric Energy Sources Biophysics Nanotechnology 02 engineering and technology Carbon nanotube Glassy carbon Pseudomonas fluorescens 010402 general chemistry 01 natural sciences law.invention Electron Transport Electron transfer Biomimetics law Electrochemistry Physical and Theoretical Chemistry Shewanella oneidensis biology Nanotubes Carbon Chemistry Cytochrome c Cytochromes c General Medicine 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Chemical engineering Biofilms biology.protein Biocomposite 0210 nano-technology |
| Zdroj: | Bioelectrochemistry. 118:131-138 |
| ISSN: | 1567-5394 |
| DOI: | 10.1016/j.bioelechem.2017.07.010 |
| Popis: | A living material was formed by self-assembly of bacterial cells ( Shewanella oneidensis MR-1 or Pseudomonas fluorescens ) with carbon nanotubes in the presence of cytochrome c from a bovine heart with the goal to mimic electroactive biofilms. The role of cytochrome c on self-assembly, cell viability and extracellular electron transfer was studied. Scanning electron microscopy and dynamic light scattering experiments highlighted its role on the self-assembly of bacteria‑carbon nanotube aggregates within only 2 h in solution. The deposition of these aggregates on glassy carbon surfaces led to a homogenous composite film in which the bacteria were embedded in a carbon nanotube network. A comparable cell density of 1 cell μm − 2 was achieved in the presence or in the absence of cytochrome c , but this protein allowed maintaining a higher bacterial viability. Electrochemical characterization demonstrated the role of cytochrome c on electron transfer reactions, leading to a current density of up to 300 μA cm − 2 in the presence of 50 mM formate when a porous carbon felt electrode is used as support for the biocomposite. |
| Databáze: | OpenAIRE |
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