Bongkrekic acid and atractyloside inhibits chloride channels from mitochondrial membranes of rat heart
| Autor: | Adam Szewczyk, Peter Stefanik, Attila Ziegelhöffer, M Ferko, Olga Krizanova, Karol Ondrias, Lubica Malekova, Viera Kominkova |
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| Rok vydání: | 2007 |
| Předmět: |
Blotting
Western Biophysics Atractyloside Mitochondrion Biochemistry chemistry.chemical_compound Chloride Channels Animals Lipid bilayer Inner mitochondrial membrane Membrane Potential Mitochondrial Dose-Response Relationship Drug Myocardium Bilayer MPTP Cell Biology Bongkrekic acid Rats Membrane chemistry Mitochondrial Membranes Chloride channel Bilayer lipid membrane Mitochondrial membrane Single channel property |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1767:31-44 |
| ISSN: | 0005-2728 |
| DOI: | 10.1016/j.bbabio.2006.10.004 |
| Popis: | The aim of this work was to characterize the effect of bongkrekic acid (BKA), atractyloside (ATR) and carboxyatractyloside (CAT) on single channel properties of chloride channels from mitochondria. Mitochondrial membranes isolated from a rat heart muscle were incorporated into a bilayer lipid membrane (BLM) and single chloride channel currents were measured in 250/50 mM KCl cis/trans solutions. BKA (1-100 microM), ATR and CAT (5-100 microM) inhibited the chloride channels in dose-dependent manner. The inhibitory effect of the BKA, ATR and CAT was pronounced from the trans side of a BLM and it increased with time and at negative voltages (trans-cis). These compounds did not influence the single channel amplitude, but decreased open dwell time of channels. The inhibitory effect of BKA, ATR and CAT on the mitochondrial chloride channel may help to explain some of their cellular and/or subcellular effects. |
| Databáze: | OpenAIRE |
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