Detection of Hepatitis B Antigen in Circulating Immune Complexes in Acute and Chronic Hepatitis
| Autor: | Paul Jungers, J. F. Bach, G. Carella, J. Drouet, G. Feldmann, M. Digeon |
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| Rok vydání: | 2008 |
| Předmět: |
Immunodiffusion
Hot Temperature Immunology Radioimmunoassay Antigen-Antibody Complex Virus Hepatitis Polyethylene Glycols Immune system Antigen Complement C1 PEG ratio medicine Humans Binding Sites Hepatitis B Surface Antigens biology business.industry Proteolytic enzymes General Medicine Hydrogen-Ion Concentration Hepatitis B medicine.disease Virology Microscopy Electron Acute Disease Chronic Disease biology.protein Antibody business Peptide Hydrolases |
| Zdroj: | Scandinavian Journal of Immunology. 6:1297-1304 |
| ISSN: | 0300-9475 |
| DOI: | 10.1111/j.1365-3083.1977.tb00369.x |
| Popis: | By using polyethylene glycol precipitation at low concentration (PEG test) and the radiolabeled Clq binding test, immune complexes were detected in sera from acute (23/28) and chronic (28/32) hepatitis patients, hemodialyzed patients with chronic hepatitis B surface (HBs) antigenemia (7/19), and asymptomatic HBs antigen carriers (2/11). After treatment of PEG precipitates with acidic pH, heating, or proteolytic enzyme (protease), electroimmunodiffusion or radioimmunoassay revealed the presence of HBs antigen or antibody in dissociated immune complexes in sera from several acute and chronic hepatitis patients. Electron microscopy showed immune complexes of HB virus in 9 of 12 PUG precipitates obtained from PEG-test-positive sera; these 9 precipitates were from patients with acute or chronic hepatitis and the other three from chronic HBs Ag carriers. Free HB virus particles were observed after protease digestion of PEG precipitates. Neither immune complexes nor virus particles were seen in precipitates from PEG-test-negative but HBs-Ag-positive sera from chronic carriers. |
| Databáze: | OpenAIRE |
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