Distribution of urocortin 2 in various tissues of the rat
| Autor: | Hideto Oono, Tamotsu Shibasaki, Naoko Yamauchi, Takahiro Nemoto, Azusa Sekino, I. Kato, Asuka Otagiri, C. Yanaihara |
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| Rok vydání: | 2005 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Corticotropin-Releasing Hormone Endocrinology Diabetes and Metabolism Central nervous system Hypothalamus Neuropeptide Ovary Biology Cellular and Molecular Neuroscience Endocrinology Internal medicine Adrenal Glands otorhinolaryngologic diseases medicine Animals RNA Messenger Rats Wistar Muscle Skeletal Lung Medulla Skin Urocortin Endocrine and Autonomic Systems Adrenal cortex Reverse Transcriptase Polymerase Chain Reaction Uterus Rats medicine.anatomical_structure Gastric Mucosa Organ Specificity Pituitary Gland Female Adrenal medulla |
| Zdroj: | Journal of neuroendocrinology. 17(10) |
| ISSN: | 0953-8194 |
| Popis: | Urocortin (Ucn) 2 is a new member of the corticotrophin-releasing hormone (CRH) neuropeptide family that is expressed in the central nervous system and peripheral tissues. However, the expression levels of Ucn 2 in various tissues of the rat remains unclear. Thus, the aim of the present study was to characterise the expression of Ucn 2 in the various tissues of the rat. Reverse transcriptase-polymerase chain reaction analysis demonstrated that Ucn 2 mRNA is expressed in the hypothalamus, pituitary, adrenal, stomach, skin, ovary, uterus and skeletal muscle. Histologically, Ucn 2 mRNA and Ucn 2-like immunoreactivity (LI) were demonstrated in both the anterior and intermediate lobes of the pituitary, but not detected in the posterior lobe. Furthermore, all Ucn 2-positive cells in the anterior and intermediate lobes were also positive for beta-endorphin. Ucn 2 mRNA was detected in the adrenal cortex and medulla although Ucn 2-LI was only found in the adrenal medulla. High-performance liquid chromatography analysis of hypothalamic, pituitary, and adrenal extracts showed that the main Ucn 2-LI peak occurred at the same molecular size as that of synthetic Ucn 2. These results suggest that Ucn 2 is synthesised in various tissues, including the anterior and intermediate lobes of the pituitary and the adrenal. |
| Databáze: | OpenAIRE |
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