A-Single Spermatogonia Heterogeneity and Cell Cycles Synchronize with Rat Seminiferous Epithelium Stages VIII–IX1
| Autor: | Karen M. Chapman, Timothy E. Richardson, F. Kent Hamra, Heather M. Powell, Jaideep Chaudhary, Priscilla Jaichander, Shadaan Abid |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Receptor ErbB-3 Cellular differentiation Population Cell Separation Biology Germline Rats Sprague-Dawley Internal medicine Testis medicine Animals Spermatogenesis education reproductive and urinary physiology education.field_of_study urogenital system Cell Cycle Cell Differentiation Articles Cell Biology General Medicine Sertoli cell Sperm Spermatogonia Rats Cell biology Seminiferous Epithelium Endocrinology medicine.anatomical_structure Reproductive Medicine Stem cell Gametogonium |
| Zdroj: | Biology of Reproduction. 90 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | In mammalian testes, ‘‘A-single’’ spermatogonia function as stem cells that sustain sperm production for fertilizing eggs. Yet, it is not understood how cellular niches regulate the developmental fate of A-single spermatogonia. Here, immunolabeling studies in rat testes define a novel population of ERBB3 + germ cells as approximately 5% of total SNAP91 + A-single spermatogonia along a spermatogenic wave. As a function of time, ERBB3 + A-single spermatogonia are detected during a 1- to 2day period each 12.9-day sperm cycle, representing 35%–40% of SNAP91 + A-single spermatogonia in stages VIII–IX of the seminiferous epithelium. Local concentrations of ERBB3 + Asingle spermatogonia are maintained under the mean density measured for neighboring SNAP91 + A-single spermatogonia, potentially indicative of niche saturation. ERBB3 + spermatogonia also synchronize their cell cycles with epithelium stages VIII–IX, where they form physical associations with preleptotene spermatocytes transiting the blood-testis barrier and Sertoli cells undergoing sperm release. Thus, A-single spermatogonia heterogeneity within this short-lived and reoccurring microenvironment invokes novel theories on how cellular niches integrate with testicular physiology to orchestrate sperm development in mammals. A-single spermatogonia, epithelial cycle, ERBB3, germline stem cell, HER3, seminiferous, SNAP91, spermatogenesis, spermatogonia, spermatogonial stem cells, stem cell niche, stem cells, testis |
| Databáze: | OpenAIRE |
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