Effect on separation of injecting samples in a solvent different from the mobile phase
| Autor: | Dorota Antos, Andreas Seidel-Morgenstern, K. Gedicke |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Threonine
Chromatography Ethanol Chemistry Organic Chemistry Analytical chemistry Water Concentration effect General Medicine Biochemistry High-performance liquid chromatography Analytical Chemistry Solvent Adsorption Models Chemical Solubility Phase (matter) Solvents Thermodynamics Computer Simulation Water content Dissolution Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 1162:62-73 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2007.02.010 |
| Popis: | The separation of compounds possessing low solubility in the mobile phase could be improved by applying stronger solvents for dissolving the feed. A model system has been investigated with ethanol-water as the mobile phase, Chirobiotic T as the stationary phase and D,L-threonine as the sample dissolved in pure water. The adsorption isotherms of D,L-threonine were determined as a function of the water content by means of a peak fitting method. Optimal injection conditions of elution chromatography were determined by process simulation. Finally, the optima obtained were verified with experimental data. |
| Databáze: | OpenAIRE |
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