Androgen signaling in decidualizing human endometrial stromal cells enhances resistance to oxidative stress
| Autor: | Osamu Ishihara, Hideno Tochigi, Satomi Uchino, Takeshi Kajihara, Japarath Prechapanich, Jan J. Brosens, Atsuo Itakura |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
endocrine system medicine.medical_specialty Stromal cell medicine.drug_class SOD2 8-Bromo Cyclic Adenosine Monophosphate Apoptosis Biology medicine.disease_cause Hysterectomy Flutamide chemistry.chemical_compound Endometrium Superoxide Dismutase-1 Internal medicine medicine Humans chemistry.chemical_classification Reactive oxygen species Leiomyoma Forkhead Box Protein O1 Superoxide Dismutase Obstetrics and Gynecology Decidualization Androgen Antagonists Dihydrotestosterone Forkhead Transcription Factors Middle Aged Androgen Prolactin Oxidative Stress Endocrinology Reproductive Medicine chemistry Premenopause Uterine Neoplasms Female Stromal Cells hormones hormone substitutes and hormone antagonists Oxidative stress medicine.drug Signal Transduction |
| Zdroj: | Fertility and sterility. 97(1) |
| ISSN: | 1556-5653 |
| Popis: | Objective To investigate the effect of androgens on the expression of genes involved in oxidative stress resistance in decidualized human endometrial stromal cells (HESCs). Design In vitro experiment. Setting University hospital. Patient(s) Premenopausal women undergoing hysterectomy for uterine fibroids. Intervention(s) Human endometrial stromal cells isolated from hysterectomy specimens were decidualized with 8-bromo-cyclic adenosine monophosphate (8-br-cAMP) and P in the presence or absence of dihydrotestosterone (DHT) at various concentrations. Hydrogen peroxide was used as a source of reactive oxygen species. Main Outcome Measure(s) Prolactin secretion, apoptosis, FOXO1, and the free radical scavengers superoxide dismutase 2 (SOD2) and SOD1 protein expression. Result(s) Prolactin production was induced in HESCs in response to 8-br-cAMP and P. Dihydrotestosterone further enhanced the secretion of PRL in cells treated with 8-br-cAMP plus P. The effect of DHT was blocked by the antiandrogen flutamide. Dihydrotestosterone enhanced resistance to oxidative stress–induced apoptosis on decidualized HESCs. Moreover, DHT enhanced FOXO1 expression in parallel with increased SOD2 protein but not with SOD1. Conclusion(s) Androgens might play a critical role in the decidualization process at the time of embryo implantation and trophoblast invasion by promoting resistance to oxidative stress. |
| Databáze: | OpenAIRE |
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