Quantification of Cytomegalovirus Glycoprotein Bn DNA in Hematopoietic Stem Cell Transplant Recipients by Real-time PCR
| Autor: | Hong Zhao, Xuan Zhang, Hai Nv Gao, Jianhua Hu, Wei Hang Ma, Xiaoming Chen, Ya Ping Huang, Jun Fan, Mei Fang Yang |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Cytomegalovirus Infection Human cytomegalovirus Viral Diseases Heredity Adolescent Clinical Research Design Science medicine.medical_treatment Genotypes Glycobiology Congenital cytomegalovirus infection Cytomegalovirus Hematopoietic stem cell transplantation Biology Real-Time Polymerase Chain Reaction Biochemistry Microbiology Viral Proteins Young Adult Immunodeficiency Viruses Virology Genotype Genetics medicine Humans Gene Glycoproteins Multidisciplinary Hematopoietic Stem Cell Transplantation virus diseases Hematopoietic stem cell medicine.disease Molecular biology Infectious Diseases Real-time polymerase chain reaction medicine.anatomical_structure Medicine Female Population Genetics Immunostaining Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 12, p e51224 (2012) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0051224 |
| Popis: | Based on sequence variation in the N-terminus of the UL55 gene, which encodes glycoprotein B (gB), human cytomegalovirus (CMV) can be classified into four gBn genotypes. We assessed the distribution of CMV gBn genotypes and the correlation between CMV gBn DNA (detected by real-time PCR) and CMV-positive pp65 cells (identified by immunohistochemical staining) in a cohort of hematopoietic stem cell transplant patients. The distribution of gB genotypes was as follows: gBn1, 60% of patients; gBn2, 13.3%; mixed gBn1 and gBn3 infection, 26.7%; and gBn4 and other mixed infections, 0%. CMV gBn1 was the most common genotype. The detected level of CMV gB DNA correlated well with the number of CMV-positive pp65 cells detected by immunostaining (r = 0.585). |
| Databáze: | OpenAIRE |
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