Efficient Monitoring of Adult and Immature Mosquitoes through Metabarcoding of Bulk Samples: A Case Study for Non-Model Culicids with Unique Ecologies
| Autor: | Ivy Luizi Rodrigues de Sá, Marcela de Paiva, Noel Fernandes Santos Neto, Dario Pires de Carvalho, Kaio Augusto Nabas Ribeiro, Maria Anice Mureb Sallum, Martha Virginia Ribeiro Rojas, Allan Kardec Ribeiro Galardo, Maria Tereza Pepe Razzolini, Jandui Almeida Amorim, Nercy Virginia Rabelo Furtado, Pedro M. Pedro |
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| Přispěvatelé: | Universidade de São Paulo (USP), IPE - Institute for Ecological Research, IEPA - Instituto de Pesquisas Cientificas e Tecnológicas do Estado do Amapá, Universidade Estadual Paulista (Unesp), Instituto Oswaldo Cruz- Rio de Janeiro, Santo Antônio Energia, Squared Biomonitoring |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
AcademicSubjects/SCI01382
0106 biological sciences 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Bulk samples Mansonia spp Animals DNA Barcoding Taxonomic AcademicSubjects/MED00860 030304 developmental biology 0303 health sciences ribosomal DNA General Veterinary biology Ecology Pupa Sampling (statistics) Sampling Distribution Dispersal biology.organism_classification mass-sampling mosquito monitoring Culicidae Infectious Diseases Taxon Larva Insect Science metabarcoding Parasitology Entomology Mansonia Brazil |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of Medical Entomology |
| Popis: | Made available in DSpace on 2021-06-25T10:31:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-01 Fundação para o Desenvolvimento da UNESP (FUNDUNESP) The rapid and economical monitoring of mosquitos is imperative to understanding the dynamics of both disease vectors and nuisance species. In light of technological advances in mosquito sampling and DNA sequencing, health agencies can now utilize the full potential of metabarcoding pipelines for rapid and standardizable surveillance. Here, we describe mosquito spatial and temporal variation, with particular focus on Mansonia Blanchard species, in the Madeira (Rondônia State) and the Ribeira (São Paulo) watersheds, Brazil using metabarcoding of the D2 rDNA marker. Sampling and molecular pipelines were used to evaluate the taxonomic contribution of mosquitos in pools of culicids collected en masse from macrophyte-roots (immatures) and from Mosquito Magnet traps and protected human landings (adults). Results for adult captures are comparable to morphological diagnoses and clarify previously unknown temporal and spatial species turnover. Metabarcoding of immature stages also confirmed the extent of the geographical distribution of some species and each taxon's association with macrophyte species. Given the benefits of metabarcoding, such as taxonomic acuity, high throughput processing, and objectivity, we suggest such techniques should be more fully incorporated into culicid monitoring schemes. The metabarcoding protocol described herein paired with standardized field sampling schemes, when used by mosquito monitoring professionals, offers substantial improvements in terms of practicality, speed and cost. Departamento de Epidemiologia Faculdade de Saúde Pública Universidade de São Paulo IPE - Institute for Ecological Research Departamento de Saúde Ambiental Faculdade de Saúde Pública Universidade de São Paulo IEPA - Instituto de Pesquisas Cientificas e Tecnológicas do Estado do Amapá FUNDUNESP - Fundação para o Desenvolvimento da UNESP Laboratório de Fisiologia e Controle de Artrópodes Vetores Instituto Oswaldo Cruz- Rio de Janeiro Santo Antônio Energia Squared Biomonitoring FUNDUNESP - Fundação para o Desenvolvimento da UNESP |
| Databáze: | OpenAIRE |
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