Overproduction of Chloroplast Glyceraldehyde-3-Phosphate Dehydrogenase Improves Photosynthesis Slightly under Elevated [CO2] Conditions in Rice
| Autor: | Eri Kondo, Mao Suganami, Keiki Ishiyama, Shinya Wada, Yuji Suzuki, Dong Kyung Yoon, Amane Makino, Chikahiro Miyake, Misaki Sugawara, Yuka Suzuki, Yuki Takegahara-Tamakawa |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Chlorophyll
Chloroplasts Physiology Ribulose-Bisphosphate Carboxylase Plant Science Photosynthesis chemistry.chemical_compound stomatognathic system Gene Expression Regulation Plant Light-independent reactions Overproduction Glyceraldehyde 3-phosphate dehydrogenase Oryza sativa biology Chemistry Ribulose Glyceraldehyde-3-Phosphate Dehydrogenases food and beverages Oryza Cell Biology General Medicine Carbon Dioxide Genetically modified rice Cytochromes f Plant Leaves Chloroplast Biochemistry biology.protein |
| Zdroj: | Plant and Cell Physiology. 62:156-165 |
| ISSN: | 1471-9053 |
| Popis: | Chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) limits the regeneration of ribulose 1,5-bisphosphate (RuBP) in the Calvin–Benson cycle. However, it does not always limit the rate of CO2 assimilation. In the present study, the effects of overproduction of GAPDH on the rate of CO2 assimilation under elevated [CO2] conditions, where the capacity for RuBP regeneration limits photosynthesis, were examined in transgenic rice (Oryza sativa). GAPDH activity was increased to 3.2- and 4.5-fold of the wild-type levels by co-overexpression of the GAPDH genes, GAPA and GAPB, respectively. In the transgenic rice plants, the rate of CO2 assimilation under elevated [CO2] conditions increased by approximately 10%, whereas that under normal and low [CO2] conditions was not affected. These results indicate that overproduction of GAPDH is effective in improving photosynthesis under elevated [CO2] conditions, although its magnitude is relatively small. By contrast, biomass production of the transgenic rice plants was not greater than that of wild-type plants under elevated [CO2] conditions, although starch content tended to increase marginally. |
| Databáze: | OpenAIRE |
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