CHID1 positively regulates RLR antiviral signaling by targeting the RIG‐I/VISA signalosome
| Autor: | Liang-Guo Xu, Sheng-Na Li, Ya-Xian Yang, Jing-Ping Huang, Ting Ling |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Proteome viruses Gene Expression Biology Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Virology Humans 030212 general & internal medicine Receptors Immunologic Adaptor Proteins Signal Transducing RIG-I Kinase Ubiquitination Pattern recognition receptor virus diseases Interferon-beta biology.organism_classification Sendai virus Cell biology HEK293 Cells Infectious Diseases Gene Knockdown Techniques Receptors Pattern Recognition DEAD Box Protein 58 030211 gastroenterology & hepatology Signal transduction Carrier Proteins IRF3 Signal Transduction Interferon regulatory factors |
| Zdroj: | Journal of Medical Virology. 91:1668-1678 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.25508 |
| Popis: | Retinoic acid-inducible gene-I (RIG-I) belongs to the RIGI-like receptors (RLRs), a class of primary pattern recognition receptors. It senses viral double-strand RNA in the cytoplasm and delivers the activated signal to its adaptor virus-induced signaling adapter (VISA), which then recruits the downstream TNF receptor-associated factors and kinases, triggering a downstream signal cascade that leads to the production of proinflammatory cytokines and antiviral interferons (IFNs). However, the mechanism of RIG-I-mediated antiviral signaling is not fully understood. Here, we demonstrate that chitinase domain-containing 1 (CHID1), a member of the chitinase family, positively regulates the RLR antiviral signaling pathway by targeting the RIG-I/VISA signalosome. CHID1 overexpression enhances the activation of nuclear factor κB (NF-кB) and interferon regulatory factor 3 (IRF3) triggered by Sendai virus (SeV) by promoting the polyubiquitination of RIG-I and VISA, thereby potentiating IFN-β production. CHID1 knockdown in human 239T cells inhibits SeV-induced activation of IRF3 and NF-κB and the induction of IFN-β. These results indicate that CHID1 positively regulates RLR antiviral signal, revealing the novel mechanism of the RIG-I antiviral signaling pathway. |
| Databáze: | OpenAIRE |
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