Calcium- and integrin-binding protein-1 is down-regulated in the sperm of patients with oligoasthenozoospermia : CIB1 expression in patients with oligoasthenozoospermia
| Autor: | Yan Yu, Wei Cui, Bin Zhang, Ji Wen, Wei Sun, Qun Guan, Qi-Yao Zhang, Zhiquan Zou, Weina Yang |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Cyclin-Dependent Kinase Inhibitor p21 Male medicine.medical_specialty chemistry.chemical_element Calcium Biology Asthenozoospermia Male infertility Pathogenesis Asian People Internal medicine Gamete Biology CDC2 Protein Kinase Genetics medicine Humans Genetics (clinical) Integrin binding Cyclin-dependent kinase 1 Obstetrics and Gynecology General Medicine Oligospermia medicine.disease Sperm Spermatozoa Cyclin-Dependent Kinases Endocrinology Reproductive Medicine chemistry Case-Control Studies Developmental Biology |
| Zdroj: | Journal of assisted reproduction and genetics. 31(5) |
| ISSN: | 1573-7330 |
| Popis: | The aim of this study was to determine whether altered expression and distribution of calcium- and integrin-binding protein-1 (CIB1) is involved in the pathogenesis of patients with oligoasthenozoospermia.Sperm samples were obtained from 25 infertile Chinese men who had failed to achieve conception after a period of 1-2 y and had been referred to the Reproductive Laboratory of the second hospital affiliated to the Shandong University of Traditional Chinese Medicine. Participants were divided into two groups: oligoasthenozoospermia (n = 13) and asthenozoospermia (n = 12); as a third group, fertile men (n = 19) were included as controls. The expression levels of mRNA and protein levels of CIB1 and cyclin-dependent kinase 1 (CDK1) were measured using qRT-PCR and western blotting.mRNA and protein expression levels of CIB1 were decreased in the oligoasthenozoospermia patients. Interestingly mRNA and protein expression levels of CDK1 were increased in the oligoasthenozoospermia patients.The results of the present study indicate that that CIB1 may be involved in the pathogenesis of oligoasthenozoospermia by the CDK1 signaling pathway. |
| Databáze: | OpenAIRE |
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