Induction of migration of periodontal ligament cells by selective regulation of integrin subunits
| Autor: | Mari Kawamura, Shinsuke Kochi, Hiroaki Aoyagi, Chiaki Yoshihara-Hirata, Kazuhiro Omori, Shogo Takashiba, Hidetaka Ideguchi, Tadashi Yamamoto, Keisuke Yamashiro |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Integrins integrin Integrin alpha3 Periodontal Ligament extracellular matrix Integrin Cell migration Extracellular matrix 03 medical and health sciences Cell Movement medicine Cell Adhesion Periodontal fiber Humans Cell adhesion Cells Cultured Cell Proliferation biology Chemistry Gene Expression Profiling Cell migration Cell Biology Original Articles microenvironment Cell biology Fibronectin 030104 developmental biology medicine.anatomical_structure periodontal ligament cells biology.protein Molecular Medicine Original Article Stem cell |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | The recruitment of tissue‐resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, coordinated by growth factors and the extracellular matrix (ECM). Integrin‐mediated cell adhesion to the ECM provides essential signals for migration. We hypothesized that PDL cell migration could be enhanced by selective expression of integrins. The migration of primary cultured PDL cells was induced by platelet‐derived growth factor‐BB (PDGF‐BB). The effects of blocking specific integrins on migration and ECM adhesion were investigated based on the integrin expression profiles observed during migration. Up‐regulation of integrins α3, α5, and fibronectin was identified at distinct localizations in migrating PDL cells. Treatment with anti‐integrin α5 antibodies inhibited PDL cell migration. Treatment with anti‐integrin α3, α3‐blocking peptide, and α3 siRNA significantly enhanced cell migration, comparable to treatment with PDGF‐BB. Furthermore, integrin α3 inhibition preferentially enhanced adhesion to fibronectin via integrin α5. These findings indicate that PDL cell migration is reciprocally regulated by integrin α3‐mediated inhibition and α5‐mediated promotion. Thus, targeting integrin expression is a possible therapeutic strategy for periodontal regeneration. |
| Databáze: | OpenAIRE |
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