Immunoengineered magnetic-quantum dot nanobead system for the isolation and detection of circulating tumor cells
| Autor: | Anwen Xiong, Huanxing Han, Mohamed Shehata Draz, Wansheng Chen, Pengfei Zhang, Wannian Yan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
genetic structures
Immunocytochemistry Magnetic nanoparticle Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Cell Separation 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology Simultaneous capture and detection Antibodies Magnetics Circulating tumor cell Magnetic isolation Antigens Neoplasm Cell Line Tumor Medical technology Humans Nanotechnology In patient Fluorescent magnetic nanobeads R855-855.5 Particle Size Magnetite Nanoparticles Volume concentration Fluorescent Dyes biology Chemistry Quantum dots Research Circulating tumor cells Maleates 021001 nanoscience & nanotechnology Epithelial Cell Adhesion Molecule Neoplastic Cells Circulating Molecular biology Peripheral blood 0104 chemical sciences Monoclonal biology.protein Molecular Medicine Polystyrenes Antibody 0210 nano-technology TP248.13-248.65 Biotechnology |
| Zdroj: | Journal of Nanobiotechnology Journal of Nanobiotechnology, Vol 19, Iss 1, Pp 1-12 (2021) |
| ISSN: | 1477-3155 |
| Popis: | Background Highly efficient capture and detection of circulating tumor cells (CTCs) remain elusive mainly because of their extremely low concentration in patients’ peripheral blood. Methods We present an approach for the simultaneous capturing, isolation, and detection of CTCs using an immuno-fluorescent magnetic nanobead system (iFMNS) coated with a monoclonal anti-EpCAM antibody. Results The developed antibody nanobead system allows magnetic isolation and fluorescent-based quantification of CTCs. The expression of EpCAM on the surface of captured CTCs could be directly visualized without additional immune-fluorescent labeling. Our approach is shown to result in a 70–95% capture efficiency of CTCs, and 95% of the captured cells remain viable. Using our approach, the isolated cells could be directly used for culture, reverse transcription-polymerase chain reaction (RT-PCR), and immunocytochemistry (ICC) identification. We applied iFMNS for testing CTCs in peripheral blood samples from a lung cancer patient. Conclusions It is suggested that our iFMNS approach would be a promising tool for CTCs enrichment and detection in one step. Graphic abstract |
| Databáze: | OpenAIRE |
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