Incorporation of monoclonal antibodies in living rat pheochromocytoma PC12 cells
| Autor: | I. D. Grozdova, P. G. Sveshnikov, Natalia K. Nagradova, N. A. Alexandrova |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cell Membrane Permeability
medicine.drug_class Protein subunit Biophysics Cyclic AMP-Dependent Protein Kinase Type II Digitonin Biology Monoclonal antibody PC12 Cells Biochemistry Antigen-Antibody Reactions chemistry.chemical_compound Structural Biology Genetics medicine Animals Protein kinase A Molecular Biology A-kinase regulatory subunit type II Antibodies Monoclonal Cell Biology respiratory system Cyclic AMP-Dependent Protein Kinases Antibody insertion Molecular biology Immune complex Rats Eukaryotic Cells chemistry PC12 cell permeabilization biology.protein CAMP binding Indicators and Reagents Antibody Intracellular Protein Binding |
| Zdroj: | FEBS Letters. 432:187-190 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(98)00861-8 |
| Popis: | PC12 cells permeabilized with a low concentration of digitonin (5 μM) under controlled conditions were loaded with monoclonal antibodies (MoAb) against the regulatory subunit type II (RII) of cAMP-dependent protein kinase. After digitonin removal from the nutrient medium (DMEM) the loaded cells repaired within 20–30 min and recontinued growth. The inserted MoAb stayed in the repaired cells at least for several hours. MoAb inhibiting the cAMP binding activity of neural RII [Grozdova et al. (1992) Biochem. Int. 27, 811–822; Sveshnikova et al. (1996) Biochem. Int. 39, 1063–1070] were shown to bind the target antigen inside the cells and influence the properties of intracellular protein kinases. |
| Databáze: | OpenAIRE |
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