Probiotic-derived ferrichrome inhibits colon cancer progression via JNK-mediated apoptosis
| Autor: | Katsuya Ikuta, Kentaro Moriichi, Nobuhiro Ueno, Yutaka Kohgo, Hiroki Tanaka, Hiroaki Akutsu, Mikihiro Fujiya, Hiroaki Konishi, Junpei Sasajima, Hiroki Tanabe |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine health care facilities manpower and services General Physics and Astronomy Apoptosis medicine.disease_cause Mass Spectrometry Transcriptome Mice chemistry.chemical_compound health care economics and organizations Ferrichrome Mice Inbred BALB C Multidisciplinary biology Kinase food and beverages Up-Regulation Cell biology Blot Lacticaseibacillus casei Treatment Outcome Colonic Neoplasms Disease Progression Fluorouracil Lactobacillus casei Cell Survival Colon MAP Kinase Signaling System Science education Mice Nude Antineoplastic Agents Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Cell Line Tumor medicine Animals Humans Gene Expression Profiling Probiotics JNK Mitogen-Activated Protein Kinases General Chemistry biology.organism_classification Molecular biology Rats 030104 developmental biology chemistry Cell culture Cisplatin Carcinogenesis |
| Zdroj: | Nature Communications, Vol 7, Iss 1, Pp 1-12 (2016) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Previous reports have suggested that some probiotics inhibit tumorigenesis and cancer progression. However, the molecules involved have not yet been identified. Here, we show that the culture supernatant of Lactobacillus casei ATCC334 has a strong tumour-suppressive effect on colon cancer cells. Using mass spectrometry, we identify ferrichrome as a tumour-suppressive molecule produced by L. casei ATCC334. The tumour-suppressive effect of ferrichrome is greater than that of cisplatin and 5-fluorouracil, and ferrichrome has less of an effect on non-cancerous intestinal cells than either of those agents. A transcriptome analysis reveals that ferrichrome treatment induces apoptosis, which is mediated by the activation of c-jun N-terminal kinase (JNK). Western blotting indicates that the induction of apoptosis by ferrichrome is reduced by the inhibition of the JNK signalling pathway. This we demonstrate that probiotic-derived ferrichrome exerts a tumour-suppressive effect via the JNK signalling pathway. Probiotics have tumour-suppressive effects in cancer cell lines and in animal models. In this study, the authors demonstrate that ferrichrome produced by Lactobacillus casei ATCC334 can suppress colon cancer growth inducing apoptosis via the JNK pathway. |
| Databáze: | OpenAIRE |
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