Adhesion of Biodegradative Anaerobic Bacteria to Solid Surfaces
| Autor: | Paula M. van Schie, Madilyn Fletcher |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Tetrachloroethylene
Surface Properties Aerobic bacteria Iron Microorganism Silicones Environmental pollution Applied Microbiology and Biotechnology Bacterial Adhesion Microbiology Bacteria Anaerobic Species Specificity Anaerobiosis Ecology biology Chemistry Adhesion biology.organism_classification Desulfomonile tiedjei Desulfovibrio Trichloroethylene Biodegradation Environmental Environmental and Public Health Microbiology Biophysics Glass Anaerobic bacteria Environmental Pollution Bacteria Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 65:5082-5088 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.65.11.5082-5088.1999 |
| Popis: | between aqueous and solid phases, as well as the environmental conditions that influence partitioning. We studied four strictly anaerobic bacteria, Desulfomonile tiedjei, Syntrophomonas wolfei, Syntrophobacter wolinii, and Desulfovibrio sp. strain G11, which theoretically together can constitute a tetrachloroethylene- and trichloroethylene-dechlorinating consortium. Adhesion of these organisms was evaluated by microscopic determination of the numbers of cells that attached to glass coverslips exposed to cell suspensions under anaerobic conditions. We studied the effects of the growth phase of the organisms on adhesion, as well as the influence of electrostatic and hydrophobic properties of the substratum. Results indicate that S. wolfei adheres in considerably higher numbers to glass surfaces than the other three organisms. Starvation greatly decreases adhesion of S. wolfei and Desulfovibrio sp. strain G11 but seems to have less of an effect on the adhesion of the other bacteria. The presence of Fe 31 on the substratum, which would be electropositive, significantly increased the adhesion of S. wolfei, whereas the presence of silicon hydrophobic groups decreased the numbers of attached cells of all species. Measurements of transport of cells through hydrophobic-interaction and electrostatic-interaction columns indicated that all four species had negatively charged cell surfaces and that D. tiedjei and Desulfovibrio sp. strain G11 possessed some hydrophobic cell surface properties. These findings are an early step toward understanding the dynamic attachment of anaerobic bacteria in anoxic environments. In polluted subsurface systems, the movement and degradation of contaminants cause changes in the environmental conditions under which subsurface microorganisms live. Numerous studies have shown that environmental factors, such as nutrient availability (23), ionic strength, and dissolved solutes (14‐18), influence the attachment of bacteria to solid surfaces. Similarly, changes in the concentration and composition of contaminants are expected to influence attachment of biodegradative bacteria in polluted subsurface environments. Understanding the significance of environmental conditions and the mechanisms by which biodegradative microorganisms partition between the aqueous and solid phases is a critical requirement for the design and evaluation of in situ bioremediation efforts. Whereas the relationship between bacterial adhesion and transport has been studied intensively with aerobic bacteria (3, 6, 14‐16, 22, 26, 33), much less is known about the adhesive |
| Databáze: | OpenAIRE |
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