A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials
| Autor: | Heidi Öblom, Erik Niemelä, Niklas Sandler, Emil Rosqvist, Ronald Österbacka, Arun P. Venu, H. Abdelkader, Jouko Peltonen, Melanie Aubert, Carl-Eric Wilén, Rajesh Koppolu, J. Frisk, Martti Toivakka, M. Mennillo, D. Soto Véliz, Petri Ihalainen, John E. Eriksson |
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| Rok vydání: | 2020 |
| Předmět: |
Paper
Scanner Materials science Biocompatibility Surface Properties Biomedical Engineering Drug Evaluation Preclinical Nanotechnology 02 engineering and technology engineering.material 03 medical and health sciences Search engine chemistry.chemical_compound Coating Coated Materials Biocompatible Materials Testing Humans General Materials Science Dimethylpolysiloxanes Particle Size Cells Cultured 030304 developmental biology Paperboard 0303 health sciences Polydimethylsiloxane General Chemistry General Medicine 021001 nanoscience & nanotechnology chemistry Pharmaceutical Preparations visual_art engineering visual_art.visual_art_medium 0210 nano-technology Science technology and society Layer (electronics) Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Rosqvist, E, Niemelä, E, Frisk, J, Öblom, H, Koppolu, R, Abdelkader, H, Véliz, D S, Mennillo, M, Venu, A P, Ihalainen, P, Aubert, M, Sandler, N, Wilén, C-E, Toivakka, M, Eriksson, J E, Österbacka, R & Peltonen, J 2020, ' A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials ', Journal of Materials Chemistry B, vol. 8, no. 6, pp. 1146-1156 . https://doi.org/10.1039/C9TB01958H |
| ISSN: | 2050-7518 |
| DOI: | 10.1039/C9TB01958H |
| Popis: | A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment. |
| Databáze: | OpenAIRE |
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