miR-320a suppresses colorectal cancer progression by targeting Rac1
| Autor: | Yingjun Quan, Daohai Zhang, Minhua Zheng, Jing Sun, Taotao Dong, Ao Huang, Lin-Lin Wang, Hongchao Zhao, Bo Feng, Houmin Zhou, Runsen Jin, Wenpeng Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
rac1 GTP-Binding Protein
Cancer Research RAC1 Biology Real-Time Polymerase Chain Reaction Cell Line Tumor microRNA medicine Humans Neoplasm Invasiveness Neoplasm Metastasis 3' Untranslated Regions Cell Proliferation DNA Primers Gene knockdown Base Sequence Three prime untranslated region Cell growth Cancer Cell migration General Medicine medicine.disease MicroRNAs Tumor progression Gene Knockdown Techniques Cancer research Disease Progression Colorectal Neoplasms |
| Zdroj: | Carcinogenesis. 35(4) |
| ISSN: | 1460-2180 |
| Popis: | MicroRNAs (miRNAs) have emerged as critical epigenetic regulators involved in cancer progression. miR-320a has been identified to be a novel tumour suppressive miRNA in colorectal cancer (CRC). However, the detailed molecular mechanisms are not fully understood. Here, we reported that miR-320a inversely associated with CRC aggressiveness in both cell lines and clinical specimens. Functional studies demonstrated that miR-320a significantly decreased the capability of cell migration/invasion and induced G0/G1 growth arrest in vitro and in vivo. Furthermore, Rac1 was identified as one of the direct downstream targets of miR-320a and miR-320a specifically binds to the conserved 8-mer at position 1140-1147 of Rac1 3'-untranslated region to regulate Rac1 protein expression. Over-expression of miR-320a in SW620 cells inhibited Rac1 expression, whereas reduction of miR-320a by anti-miR-320a in SW480 cells enhanced Rac1 expression. Re-expression of Rac1 in the SW620/miR-320a cells restored the cell migration/invasion inhibited by miR-320a, whereas knockdown of Rac1 in the SW480/anti-miR-320a cells repressed these cellular functions elevated by anti-miR-320a. Conclusively, our results demonstrate that miR-320a functions as a tumour-suppressive miRNA through targeting Rac1 in CRC. |
| Databáze: | OpenAIRE |
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