A simple and sensitive liquid chromatographic method for the analysis of free docosanoic, tetracosanoic and hexacosanoic acids in human plasma as fluorescent derivatives
| Autor: | Mei-Chyn Chao, Tang-Chia Chung, Ying-Ju Ou, Hwang-Shang Kou, Hsin-Lung Wu |
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| Rok vydání: | 2007 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities endocrine system Spectrometry Mass Electrospray Ionization endocrine system diseases Electrospray ionization Fluorescence spectrometry Biochemistry High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry chemistry.chemical_compound Environmental Chemistry Humans Sample preparation Derivatization Spectroscopy Chromatography High Pressure Liquid Fluorescent Dyes Detection limit Chromatography Molecular Structure Fatty Acids Reference Standards Toluene chemistry Reagent |
| Zdroj: | Analytica chimica acta. 611(1) |
| ISSN: | 1873-4324 |
| Popis: | Docosanoic (C22), tetracosanoic (C24) and hexacosanoic (C26) acids are saturated very-long-chain fatty acids (VLCFA) present at trace levels in biosamples. VLCFA can be used as potential biomarkers for the diagnosis of hereditary diseases such as X-linked adrenoleukodystrophy. Because the analytes to be detected are at trace levels, a sensitive fluorimetric liquid chromatographic method was developed to analyze VLCFA in plasma. The method is simple based on extracting VLCFA from plasma with toluene, and the obtained toluene extract was subject to the derivatization of VLCFA with a fluorescent reagent 2-(2-naphthoxy)ethyl-2-(piperidino)ethanesulfonate (NOEPES) without solvent evaporation/replacement. The resulting fluorescent derivatives were monitored by fluorimetric detection (excitation at 225 nm and emission at 360 nm), giving a high sensitivity with the limit of detection about 5.0 nM (S/N = 3, 10 μL injected) of the analytes. Application of the method to the analysis of VLCFA in the plasma of patients with adrenoleukodystrophy proved practical and effective. |
| Databáze: | OpenAIRE |
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