Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein

Autor: Xiaoying Zhang, Xiang Liu, Fagang Zhong, Long Xu, Ben Li, Shikun Ge
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Parvovirus
Canine
040301 veterinary sciences
T7 phage
animal diseases
viruses
[SDV]Life Sciences [q-bio]
Immunoglobulins
Enzyme-Linked Immunosorbent Assay
chemical and pharmacologic phenomena
Antibodies
Viral
Virus
0403 veterinary science
Parvoviridae Infections
IgY-Technology
03 medical and health sciences
Dogs
virus diseases
medicine
Animals
Dog Diseases
lcsh:Veterinary medicine
General Veterinary
biology
Canine distemper
Parvovirus
parvovirus
Canine parvovirus
Canine coronavirus
04 agricultural and veterinary sciences
respiratory system
infectious animal diseases
medicine.disease
biology.organism_classification
Virology
Antibodies
Neutralizing
3. Good health
Molecular Docking Simulation
Titer
030104 developmental biology
Capsid
lcsh:SF600-1100
Capsid Proteins
Female
Chickens
phage display system
Research Article
Single-Chain Antibodies
Zdroj: Veterinary Research
Veterinary Research, BioMed Central, 2020, 51 (1), pp.110. ⟨10.1186/s13567-020-00832-7⟩
Veterinary Research, Vol 51, Iss 1, Pp 1-11 (2020)
ISSN: 0928-4249
1297-9716
DOI: 10.1186/s13567-020-00832-7⟩
Popis: Canine parvovirus (CPV) can cause acute and highly contagious bloody enteritis in dog. To obtain antibodies against CPV, hens were immunized with virus-like particles (VLP) of CPV-VP2. The IgY single chain fragment variables (scFv) were generated by T7 phage display system and expressed in E. coli system. The titer of the primary scFv library reached to 1.5 × 106 pfu/mL, and 95% of the phages contained the target fragments. The CPV-VLP and CPV-VP2 protein showed similar reaction values to the purified scFv in the ELISA test, and the results of ELISA analysis using IgY-scFv toward CPV clinical samples were consistent with commercial immunochromatographic assay (ICA) and PCR detection, the scFv did not show cross reactivity with canine distemper virus (CDV) and canine coronavirus (CCV). IgY-scFv was successfully expressed in CRFK cells, and in the virus suppression assay, 55% of CPV infections were eliminated within 24 h. Docking results demonstrated that the number of amino acids of the binding sides between scFv and VP2 were AA37 and AA40, respectively. This study revealed the feasibility of a novel functional antibody fragment development strategy by generating diversified avian IgY-scFv libraries towards the pathogenic target of interest for both detection and therapeutic purposes in veterinary medicine.
Databáze: OpenAIRE
Externí odkaz: