THE BUFFERED PERFORMIC-ACID-ALCIAN-BLUE-PERIODIC-ACID-SCHIFF METHOD FOR THE DIFFERENTIATION OF BASOPHILS IN THE HUMAN AND RAT PITUITARY
| Autor: | K. Swettenham |
|---|---|
| Rok vydání: | 1960 |
| Předmět: |
Male
Formates Periodic acid–Schiff stain Basophil Biology Pathology and Forensic Medicine Leukocyte Count chemistry.chemical_compound Pituitary Gland Anterior medicine Animals Humans Coloring Agents Fixation (histology) Performic acid Staining and Labeling Histological Techniques Cell Differentiation Articles General Medicine Basophils Rats Staining Solvent Rat Pituitary medicine.anatomical_structure Biochemistry chemistry Pituitary Gland Alcian Blue |
| Zdroj: | Journal of Clinical Pathology. 13:256-260 |
| ISSN: | 0021-9746 |
| DOI: | 10.1136/jcp.13.3.256 |
| Popis: | The difficulty of using a single method for staining rat and human pituitary was investigated by testing separately and in different combinations the effects of the various components in the performic-acid-alcian-blue(P.F.A.B.)-periodic-acid-Schiff(P.A.S.) method (Adams and Swettenham, 1958). In this way the possibility of inhibiting or enhancing by one procedure the staining effects of the other could be assessed. The profound influence of fixation on these staining methods was demonstrated by employing the same set of staining methods on tissues fixed in different ways. The possibility that unbuffered performic acid might exert a considerable solvent action on certain proteins was considered to be related to the difficulty of applying the P.F.A.B.-P.A.S. method to the rat pituitary. Buffered performic acid has far less solvent action and the P.F.A.B.-P.A.S. method, modified by the replacement of buffered for unbuffered performic acid, proved to be successful in the demonstration of two types of basophil cells in both rat and man. |
| Databáze: | OpenAIRE |
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