The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: effects of obesity, insulin resistance, and TZD treatment
Autor: | Beata Lecka-Czernik, Neda Rasouli, Resat Unal, Gouri Ranganathan, Aiwei Yao-Borengasser, Irina D. Pokrovskaya, Philip A. Kern, Bounleut Phanavanh |
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Rok vydání: | 2006 |
Předmět: |
Adult
Male medicine.medical_specialty medicine.medical_treatment fatty acid synthetase Adipose tissue thiazolidinedione QD415-436 Biology diacylglycerol transferase Biochemistry Gene Expression Regulation Enzymologic Article Impaired glucose tolerance Mice chemistry.chemical_compound Endocrinology Insulin resistance Internal medicine Adipocytes medicine Animals Humans Hypoglycemic Agents Insulin Diacylglycerol O-Acyltransferase Obesity Aged Pioglitazone Triglyceride nutritional and metabolic diseases Cell Biology Middle Aged medicine.disease Mice Inbred C57BL Lipoprotein Lipase Adipose Tissue Lipotoxicity chemistry Female Thiazolidinediones lipids (amino acids peptides and proteins) Fatty Acid Synthases Insulin Resistance Rosiglitazone medicine.drug |
Zdroj: | Journal of Lipid Research, Vol 47, Iss 11, Pp 2444-2450 (2006) |
ISSN: | 0022-2275 |
Popis: | Acyl-coenzyme A:diacylglycerol transferase (DGAT), fatty acid synthetase (FAS), and LPL are three enzymes important in adipose tissue triglyceride accumulation. To study the relationship of DGAT1, FAS, and LPL with insulin, we examined adipose mRNA expression of these genes in subjects with a wide range of insulin sensitivity (SI). DGAT1 and FAS (but not LPL) expression were strongly correlated with SI. In addition, the expression of DGAT1 and FAS (but not LPL) were higher in normal glucose-tolerant subjects compared with subjects with impaired glucose tolerance (IGT) (P < 0.005). To study the effects of insulin sensitizers, subjects with IGT were treated with pioglitazone or metformin for 10 weeks, and lipogenic enzymes were measured in adipose tissue. After pioglitazone treatment, DGAT1 expression was increased by 33 +/- 10% (P < 0.05) and FAS expression increased by 63 +/- 8% (P < 0.05); however, LPL expression was not altered. DGAT1, FAS, and LPL mRNA expression were not significantly changed after metformin treatment. The treatment of mice with rosiglitazone also resulted in an increase in adipose expression of DGAT1 by 2- to 3-fold, as did the treatment of 3T3 F442A adipocytes in vitro with thiazolidinediones. These data support a more global concept suggesting that adipose lipid storage functions to prevent peripheral lipotoxicity. |
Databáze: | OpenAIRE |
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