Neural precursor cells inhibit multiple inflammatory signals
| Autor: | S.Z. Ben Sasson, Michal Baniyash, Nina Fainstein, Ilan Vaknin, Tamir Ben-Hur, Philip Zisman, Ofira Einstein |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
T cell T-Lymphocytes chemical and pharmacologic phenomena Biology Lymphocyte Activation Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Immune system Downregulation and upregulation Precursor cell Internal medicine otorhinolaryngologic diseases medicine Animals Molecular Biology Cells Cultured Cell Proliferation Inflammation Neurons Interleukin-6 Stem Cells Experimental autoimmune encephalomyelitis T-cell receptor Janus Kinase 3 Cell Biology medicine.disease Coculture Techniques Cell biology Mice Inbred C57BL stomatognathic diseases Endocrinology medicine.anatomical_structure chemistry Apoptosis Ionomycin Interleukin-2 Female Lymph Nodes Signal Transduction |
| Zdroj: | Molecular and cellular neurosciences. 39(3) |
| ISSN: | 1095-9327 |
| Popis: | Intravenous neural precursor cell (NPCs) injection attenuates experimental autoimmune encephalomyelitis by reducing autoreactive T cell encephalitogenicity in lymph nodes in vivo. Here we examined NPC-lymphocyte interactions in vitro. NPCs inhibited the induction of T cell activation marker IL-2-Receptor alpha, ICOS, PD-1 and CTLA-4 and inhibited T cell proliferation. NPCs inhibited T cell activation and proliferation in response to Concavalin-A and to anti-CD3/anti-CD28, which are T cell receptor (TCR)-mediated stimuli, but not in response to phorbol myristate acetate/ionomycin, a TCR-independent stimulus. The suppressive effect was not mediated via downregulation of CD3epsilon or induction of apoptosis. We next examined NPCs effects on inflammatory-cytokine signaling. NPCs impaired IL-2-mediated phosphorylation of JAK3 in lymphocytes, and inhibited IL-6 mediated proliferation of B9 murine hybridoma cells. In conclusion, NPCs ameliorate TCR-mediated T cell activation and inhibit inflammatory cytokines' signaling in immune cells. These findings may underlie the broad anti-inflammatory effects of NPCs in vivo. |
| Databáze: | OpenAIRE |
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