Analysis of Donor- and Isolation-Related Variables from Non-Heart-Beating Donors (NHBDs) Using the Kyoto Islet Isolation Method
| Autor: | Nobuyo Hatanaka, Chunfang Song, Shinichi Matsumoto, Shuichi Miyakawa, Teru Okitsu, Hideo Nagata, Hirofumi Noguchi, Naoya Kobayashi, Yukihide Yonekawa, Yasuhiro Iwanaga, Xiaoling Liu, M. Ueda, Hiroki Kamiya |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male endocrine system endocrine system diseases Isolation (health care) Islets of Langerhans Transplantation Biomedical Engineering lcsh:Medicine Cell Separation Body Mass Index Andrology Risk Factors Diabetes mellitus medicine Humans Transplantation Type 1 diabetes geography geography.geographical_feature_category business.industry lcsh:R Body Weight Cell Biology Middle Aged medicine.disease Islet Tissue Donors Diabetes Mellitus Type 1 Tissue and Organ Harvesting Female Pancreatic islet transplantation business |
| Zdroj: | Cell Transplantation, Vol 17 (2008) |
| ISSN: | 1555-3892 0963-6897 |
| DOI: | 10.3727/096368908786092711 |
| Popis: | Recently, we demonstrated that islet transplantation from non-heart-beating donors (NHBDs) using the Kyoto islet isolation method (KIIM) successfully reversed patients' diabetes state. In this study, we evaluated the effects of donor- and isolation-related variables on islet isolation results from NHBDs by KIIM. Twenty-one islet preparations from the pancreata of NHBDs were isolated by KIIM. Islet preparations that met transplantation criteria and achieved improved patient diabetes control after transplantation were defined as successful isolations. Potential risk factors deemed to affect islet isolation results, such as age, gender, body mass index, hospital stay, donors' blood biochemical tests, a modified pancreata procurement method, and isolation and purification procedure-related variables, were analyzed. Seventeen out of 21 islet isolations (81%) were successful isolations. Postpurification islet yield was 447,639 ± 39,902 islet equivalents (IE) in the successful isolation group and 108,007 ± 31,532 IE in the failure group. Donor age was significantly younger in the success group (41.9 ± 4.0 years old in the success group vs. 57.5 ± 2.2 years old in the failure group, p = 0.003). Chronic pancreatitis significantly decreased islet yields (p = 0.006). Phase I time was significantly shorter (p = 0.010) and undigested tissue volume was significantly smaller (p = 0.020) in the success group. Purity was in positive correlation to postpurification islet yield, while donor age was in reverse correlation to postpurification islet yield. KIIM enables us to perform islet transplantation from NHBDs; however, the decision to use pancreata from older donors or those with chronic pancreatitis requires careful consideration. |
| Databáze: | OpenAIRE |
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