The peptide secreted at the water to land transition in a model amphibian has antioxidant effects
Autor: | Sonia N Báo, Augusto Batagin-Neto, Lucas Alverne Freitas de Albuquerque, Anderson Dematei, Filipe C. D. A. Lima, Selma A S Kuckelhaus, Felipe Saldanha-Araujo, Guilherme D. Brand, Antonio Sebben, Daniel C. Moreira, Cátia Teixeira, Wanessa Felix Cabral, Eder Alves Barbosa, Paula Gomes, Tatiana Karla dos Santos Borges, Alexandra Plácido, José Roberto S. A. Leite, Peter Eaton, Renato Socodato, Amandda Évelin Silva-Carvalho, Camila C. Portugal, João B. Relvas, Massuo J. Kato |
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Přispěvatelé: | Universidade de Brasília (UnB), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), UPTEC, Universidade Do Porto, Ciência e Tecnologia de São Paulo, Universidade Estadual Paulista (UNESP), University of Lincoln, Universidade de São Paulo (USP) |
Rok vydání: | 2021 |
Předmět: |
Pithecopus azureus
Triptofilinas Amphibian Ontogeny In silico media_common.quotation_subject Cell tryptophyllin Peptide Anfíbio Antioxidants General Biochemistry Genetics and Molecular Biology Amphibia Neuroblast Live cell imaging biology.animal medicine oxidative stress Animals Humans Stress oxidativo Metamorphosis Skin General Environmental Science media_common Mammals chemistry.chemical_classification Peptídeos General Immunology and Microbiology biology Water General Medicine Espectrometria de massa Cell biology antioxidant peptide medicine.anatomical_structure chemistry MALDI mass spectrometry imaging Anura Biological Applications Peptides General Agricultural and Biological Sciences |
Zdroj: | Proc Biol Sci Repositório Institucional da UnB Universidade de Brasília (UnB) instacron:UNB Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
ISSN: | 1471-2954 0962-8452 |
DOI: | 10.1098/rspb.2021.1531 |
Popis: | Made available in DSpace on 2022-04-29T08:36:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O 2 levels and UV exposure on land compared with aquatic environments. Laboratório de Síntese e Análise de Biomoléculas Instituto de Química Universidade de Brasília Núcleo de Pesquisa em Morfologia e Imunologia Aplicada (NuPMIA) Faculdade de Medicina Universidade de Brasília Laboratório de Imunologia Celular NuPMIA Faculdade de Medicina Universidade de Brasília Programa de Pós-graduação em Medicina Tropical Núcleo de Medicina Tropical Faculdade de Medicina Universidade de Brasília Laboratório de Hematologia e Celulas-tronco Faculdade de Ciências da Saúde Universidade de Brasília Laboratório de Microscopia e Microanálise Instituto de Ciências Biológicas Universidade de Brasília Instituto de Ciências Biológicas Universidade de Brasília Laboratório de Espectrometria de Massa Embrapa Recursos Genéticos e Biotecnologia Bioprospectum Lda UPTEC LAQV/REQUIMTE Departamento de Química e Bioquímica Faculdade de Ciências Universidade Do Porto Glial Cell Biology Lab Instituto de Investigacao e Inovacao em Saude and Instituto de Biologia Molecular e Celular Universidade Do Porto Instituto Federal de Educação Ciência e Tecnologia de São Paulo São Paulo State University (Unesp) Campus of Itapeva The Bridge School of Chemistry Joseph Banks Laboratories University of Lincoln Departamento de Química Fundamental Instituto de Química Universidade de São Paulo, SP São Paulo State University (Unesp) Campus of Itapeva |
Databáze: | OpenAIRE |
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