Autoantibodies in sclerosing cholangitis against a shared peptide in biliary and colon epithelium
| Autor: | Linda Squillante, Rajender Reddy, Eugene R. Schiff, Jeffers Lj, Aditya Mandal, Arunansu Dasgupta, Kiron M. Das, Shakir A. Hyder |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology endocrine system diseases Colon Biliary Tract Diseases Cholangitis Sclerosing Enzyme-Linked Immunosorbent Assay digestive system Gastroenterology Immunoglobulin G Epithelium Primary sclerosing cholangitis Immunoenzyme Techniques Epitopes Primary biliary cirrhosis Internal medicine medicine Humans Aged Autoantibodies Sclerosis Hepatology biology business.industry Bile duct Tissue Extracts Gallbladder digestive oral and skin physiology Autoantibody Antibodies Monoclonal Middle Aged medicine.disease digestive system diseases medicine.anatomical_structure Blood Biliary tract biology.protein Female Bile Ducts Antibody business Peptides |
| Zdroj: | Gastroenterology. 106(1) |
| ISSN: | 0016-5085 |
| Popis: | Background/Aims: A strong association exists between ulcerative colitis and primary sclerosing cholangitis (PSC). Previously, the presence of a unique epitope shared by colon and biliary epithelial cells was shown by using the novel monoclonal antibody (MAb) 7E12H12 developed against a colonic epithelial protein. In the present study, the presence of circulating autoantibody in PSC against this peptide was examined. Methods: Sera from 16 patients with PSC, 13 with primary biliary cirrhosis, 6 with secondary biliary stricture, and 6 with chronic liver diseases and 10 normal subjects were used. An inhibition immunoperoxidase assay using the 7E12H12 MAb was developed against sections of bile duct and gallbladder. Sera were also examined in an enzyme-linked immunosorbent assay (ELISA) against the gallbladder extract enriched in 7E12H12-reactive protein. Results: About two thirds of the sera from patients with PSC blocked the binding of 7E12H12 MAb on the bile duct and gallbladder, whereas non-PSC sera did not. In the ELISA, 93% of PSC sera had circulating immunoglobulin G antibodies against the enriched gallbladder extract. The reactivity of sera from the PSC group was significantly (P Conclusions: Sera from patients with PSC contains autoantibodies against a cross-reactive peptide shared by colon and biliary epithelial cells. |
| Databáze: | OpenAIRE |
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