Clonal and Quantitative In Vivo Assessment of Hematopoietic Stem Cell Differentiation Reveals Strong Erythroid Potential of Multipotent Cells

Autor: Praveen K. Muthuswamy, Herman Tsang, Anna E. Beaudin, Mark Landon, Scott W. Boyer, E. Camilla Forsberg, Eric W. Martin, Stephanie Smith-Berdan, Christa Cheung, Jessica Perez-Cunningham, Smrithi Rajendiran
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Myeloid
Regenerative Medicine
Biochemistry
Mice
0302 clinical medicine
Models
Stem Cell Research - Nonembryonic - Human
Erythropoiesis
lcsh:QH301-705.5
clonal analysis
lcsh:R5-920
Hematopoietic stem cell differentiation
Hematopoietic Stem Cell Transplantation
Cell Differentiation
Hematology
3. Good health
Cell biology
Haematopoiesis
Blood
medicine.anatomical_structure
multilineage differentiation
Stem Cell Research - Nonembryonic - Non-Human
Stem cell
hematopoietic differentiation
lcsh:Medicine (General)
1.1 Normal biological development and functioning
Clinical Sciences
Spleen
Biology
Models
Biological
Article
Immunophenotyping
Colony-Forming Units Assay
03 medical and health sciences
Genetics
medicine
single-cell transplantation
Animals
Inflammatory and Immune System
Cell Lineage
Progenitor cell
quantitative analyses
lineage potential
Transplantation
Multipotent Stem Cells
Cell Biology
Biological
Stem Cell Research
Hematopoietic Stem Cells
hematopoietic stem cells
Hematopoiesis
030104 developmental biology
lcsh:Biology (General)
reconstitution
Biochemistry and Cell Biology
heterogeneity
030217 neurology & neurosurgery
erythropoiesis
Biomarkers
Developmental Biology
Zdroj: Stem Cell Reports, Vol 12, Iss 4, Pp 801-815 (2019)
Stem Cell Reports
Boyer, Scott W; Rajendiran, Smrithi; Beaudin, Anna E; Smith-Berdan, Stephanie; Muthuswamy, Praveen K; Perez-Cunningham, Jessica; et al.(2019). Clonal and Quantitative In Vivo Assessment of Hematopoietic Stem Cell Differentiation Reveals Strong Erythroid Potential of Multipotent Cells.. Stem cell reports. doi: 10.1016/j.stemcr.2019.02.007. UC Santa Cruz: Retrieved from: http://www.escholarship.org/uc/item/2w80n4jg
Stem cell reports, vol 12, iss 4
ISSN: 2213-6711
Popis: Summary Hematopoiesis is arguably one of the best understood stem cell systems; however, significant challenges remain to reach a consensus understanding of the lineage potential, heterogeneity, and relationships of hematopoietic stem and progenitor cell populations. To gain new insights, we performed quantitative analyses of mature cell production from hematopoietic stem cells (HSCs) and multiple hematopoietic progenitor populations. Assessment of the absolute numbers of mature cell types produced by each progenitor cell revealed a striking erythroid dominance of all myeloid-competent progenitors assessed, accompanied by strong platelet reconstitution. All populations with myeloid potential also produced robust numbers of red blood cells and platelets in vivo. Clonal analysis by single-cell transplantation and by spleen colony assays revealed that a significant fraction of HSCs and multipotent progenitors have multilineage potential at the single-cell level. These new insights prompt an erythroid-focused model of hematopoietic differentiation.
Graphical Abstract
Highlights • RBCs are the predominant cell type produced by multipotent hematopoietic progenitors • All cell types with myeloid potential also produced RBCs and platelets in vivo • Single HSCs and MPPF cells are capable of multilineage hematopoietic reconstitution • Erythroid cell production emerges as a default hematopoietic fate
In this article, Forsberg and colleagues use quantitative reconstitution assays to demonstrate that all myeloid-competent progenitor cells also contribute to erythroid and platelet production. Single-cell in vivo analyses showed that hematopoietic stem cells and multipotent progenitors are clonally multipotent. These results suggest that erythropoiesis is a default hematopoietic fate and challenges current views on hematopoietic lineage potential and fate decisions.
Databáze: OpenAIRE
Externí odkaz: