Gonadotropin-dependent regulation of the prostaglandin E2 receptor in equine preovulatory follicles during the ovulatory process in mares
| Autor: | Khampoune Sayasith, Monique Doré, Jean Sirois, Nadine Bouchard |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Ovulation
endocrine system medicine.medical_specialty DNA Complementary medicine.drug_class media_common.quotation_subject Molecular Sequence Data Ovary Biology Human chorionic gonadotropin Ovarian Follicle Internal medicine Follicular phase Genetics medicine Animals Receptors Prostaglandin E Amino Acid Sequence Horses Cloning Molecular Ovarian follicle DNA Primers media_common Base Sequence Reverse Transcriptase Polymerase Chain Reaction urogenital system Theca interna Sequence Analysis DNA Cell Biology Immunohistochemistry Blotting Southern medicine.anatomical_structure Endocrinology Gene Expression Regulation Theca Female Gonadotropin Gonadotropins Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 76:191-201 |
| ISSN: | 1098-2795 1040-452X |
| Popis: | The objectives of the study were to clone the primary structure of the prostaglandin E2 receptor subtype 2 (PTGER2) cDNA and to characterize its regulation in equine follicles during gonadotropin-induced ovulation. Results from DNA isolation indicated that the equine PTGER2 cDNA encodes a predicted 353-amino acid protein, which is highly similar (76–85%) to known mammalian homologues. The regulation of PTGER2 was studied by semi-quantitative RT-PCR/Southern blot using preparations of theca interna and mural granulosa cells isolated from equine follicles 0–39 hr post-treatment with human chorionic gonadotropin (hCG). Results indicated that a significant increase of PTGER2 mRNA occurred at 24 and 39 hr post-hCG in granulosa cells, and 30 and 33 hr post-hCG in theca cells (P |
| Databáze: | OpenAIRE |
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