| Autor: |
Owen J. T. McCarty, Michelle A. Berny, Tara C. White-Adams, Andras Gruber, Ishan Patel, David Gailani, Erik I. Tucker |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Journal of Thrombosis and Haemostasis. 8:1295-1301 |
| ISSN: |
1538-7836 |
| DOI: |
10.1111/j.1538-7836.2010.03850.x |
| Popis: |
Summary. Background: Laminin is the most abundant non-collagenous protein in the basement membrane. Recent studies have shown that laminin supports platelet adhesion, activation and aggregation under flow conditions, highlighting a possible role for laminin in hemostasis. Objective: To investigate the ability of laminin to promote coagulation and support thrombus formation under shear. Results and methods: Soluble laminin accelerated factor (F) XII activation in a purified system, and shortened the clotting time of recalcified plasma in a FXI- and FXII-dependent manner. Laminin promoted phosphatidylserine exposure on platelets and supported platelet adhesion and fibrin formation in recalcified blood under shear flow conditions. Fibrin formation in laminin-coated capillaries was abrogated by an antibody that interferes with FXI activation by activated FXII, or an antibody that blocks activated FXI activation of FIX. Conclusion: This study identifies a role for laminin in the initiation of coagulation and the formation of platelet-rich thrombi under shear conditions in a FXII-dependent manner. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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