Codetection of a Mixed Population ofcandHPV62 Containing Wild‐Type and Disrupted E1 Open‐Reading Frame in a 45‐Year‐Old Woman with Normal Cytology
| Autor: | Robert D. Burk, Masanori Terai, Leiping Fu, Toshihiko Matsukura, Rolando Herrero |
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| Rok vydání: | 2004 |
| Předmět: |
viruses
Molecular Sequence Data Population Cervix Uteri Genome Viral Biology medicine.disease_cause Genome law.invention Open Reading Frames law medicine Humans Immunology and Allergy Cloning Molecular ORFS education Papillomaviridae Polymerase chain reaction Cloning Genetics education.field_of_study Mutation Base Sequence Wild type virus diseases Middle Aged Virology Open reading frame Infectious Diseases Female |
| Zdroj: | The Journal of Infectious Diseases. 190:1303-1309 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/423855 |
| Popis: | We have cloned, sequenced, and characterized the complete genome of a novel human papillomavirus (HPV), candHPV62. During cloning, 2 candHPV62 viral isolates were recovered from a single cervical sample; 1 had all anticipated HPV open-reading frames (ORFs) intact, whereas the other exhibited an E1 frame-shift mutation. Further experiments indicated that the 2 strains were equivalent in abundance. It appears that an early mutation occurred within the E1 ORF, which was transcomplemented by an intact E1 protein. A search of the HPV database identified disruption of the E1 ORF in the cloned reference isolates of HPV16, HPV53, HPV56, and HPV72. These data suggest that disruption of the E1 ORF in genital HPVs is not uncommon. |
| Databáze: | OpenAIRE |
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