Kinetochore microtubule dynamics and attachment stability are regulated by Hec1
| Autor: | Andrea Musacchio, Daniela Cimini, Walter E. Gall, Edward D. Salmon, Claudio Ciferri, Jennifer G. DeLuca |
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| Rok vydání: | 2006 |
| Předmět: |
Biorientation
Kinesins Spindle Apparatus Biology Protein Serine-Threonine Kinases Microtubules Models Biological General Biochemistry Genetics and Molecular Biology Ndc80 complex Spindle pole body Kinetochore microtubule Biopolymers Microtubule Aurora Kinases Centromere Aurora Kinase B Humans Amino Acids Phosphorylation Kinetochores Metaphase Biochemistry Genetics and Molecular Biology(all) Kinetochore Antibodies Monoclonal Nuclear Proteins Cell biology NDC80 Cytoskeletal Proteins Protein Transport Biologie Microtubule-Associated Proteins Epitope Mapping HeLa Cells |
| Zdroj: | Cell. 127(5) |
| ISSN: | 0092-8674 |
| Popis: | SummaryMitotic cells face the challenging tasks of linking kinetochores to growing and shortening microtubules and actively regulating these dynamic attachments to produce accurate chromosome segregation. We report here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability. Microinjection of an antibody to the N terminus of Hec1 suppresses both microtubule detachment and microtubule plus-end polymerization and depolymerization at kinetochores of PtK1 cells. Centromeres become hyperstretched, kinetochore fibers shorten from spindle poles, kinetochore microtubule attachment errors increase, and chromosomes severely mis-segregate. The N terminus of Hec1 is phosphorylated by Aurora B kinase in vitro, and cells expressing N-terminal nonphosphorylatable mutants of Hec1 exhibit an increase in merotelic attachments, hyperstretching of centromeres, and errors in chromosome segregation. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules. |
| Databáze: | OpenAIRE |
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