A novel role for E- and P-selectins: shape control of endothelial cell monolayers
| Autor: | Anne-Marie Benoliel, Pierre Bongrand, Catherine Farnarier, S. Kaplanski, Colette Foa, Gilles Kaplanski |
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| Rok vydání: | 1994 |
| Předmět: |
Umbilical Veins
Endothelium Intercellular Adhesion Molecule-1 Inflammation Platelet Membrane Glycoproteins Biology Calcium in biology Cell Movement von Willebrand Factor E-selectin Cell Adhesion Leukocytes medicine Humans Cells Cultured Cell Size Cell adhesion molecule Infant Newborn Thrombin Antibodies Monoclonal Cell Biology Cell biology Endothelial stem cell P-Selectin Intercellular Junctions medicine.anatomical_structure Immunology biology.protein Calcium Endothelium Vascular medicine.symptom E-Selectin Cell Adhesion Molecules Selectin Interleukin-1 Signal Transduction |
| Zdroj: | Journal of Cell Science. 107:2449-2457 |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | The migration of neutrophils from blood vessels to peripheral tissues is a key step of inflammation. This requires the formation of transient gaps between endothelial cells with concomitant leucocyte squeezing through these narrow apertures and immediate restoration of endothelium continuity. It is currently considered that the main role of selectins is to mediate the initial contact between flowing leucocytes and endothelial cells. We show here that the binding of E- or P-selectins by specific antibodies induces a marked ‘rounding up’ of interleukin-1- or thrombin-activated human endothelial cells, respectively. Also, anti-E-selectin antibodies trigger a transient increase in cytosolic calcium involving intracellular calcium stores. No such effect is observed when von Willebrand factor or intercellular adhesion molecule 1 are similarly bound. Thus, in addition to promoting the initial interaction between activated endothelium and moving leucocytes, selectins might play a role in the induction of subsequent endothelial deformation, which would facilitate leucocyte arrest and transmigration towards peripheral tissues, and enhance the diffusion of soluble molecules between intravascular and peripheral compartments. Our results are consistent with this hypothesis and demonstrate a new property of endothelial selectins. |
| Databáze: | OpenAIRE |
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