A Ewing's Sarcoma Cell Line Showing Some, but Not All, of the Traits of a Cholinergic Neuron
| Autor: | Seana O'Regan, François-Marie Meunier, Sheela Vyas, Marie-Françoise Diebler |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Vesamicol Tyrosine 3-Monooxygenase Proteolipids Nerve Tissue Proteins Sarcoma Ewing Biology Tritium Biochemistry Synaptic vesicle Choline Choline O-Acetyltransferase Synaptotagmins Cellular and Molecular Neuroscience chemistry.chemical_compound Piperidines Acetylcholine transport Tumor Cells Cultured medicine Humans RNA Messenger Cholinergic neuron Neurotransmitter Neurons Membrane Glycoproteins Calcium-Binding Proteins Choline acetyltransferase Acetylcholine Cell biology Cholinergic Fibers chemistry Neuromuscular Depolarizing Agents Synaptotagmin I Cholinergic medicine.drug |
| Zdroj: | Journal of Neurochemistry. 64:69-76 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1046/j.1471-4159.1995.64010069.x |
| Popis: | The Ewing's sarcoma cell line ICB 112 was examined in detail for a cholinergic phenotype. Choline acetyltransferase activity (12.3 +/- 2.9 nmol/h/mg of protein) was associated with the presence of multiple mRNA species labeled with a human choline acetyltransferase riboprobe. Choline was taken up by the cells by a high-affinity, hemicholinium-3-sensitive transporter that was partially inhibited when lithium replaced sodium in the incubation medium; the choline taken up was quickly incorporated into both acetylcholine and phosphorylcholine. High-affinity binding sites for vesamicol, an inhibitor of vesicular acetylcholine transport, were also present. The mRNAs for synaptotagmin (p65) and the 15-kDa proteolipid were readily detected and were identical in size to those observed in cholinergic regions of the human brain. Cumulative acetylcholine efflux was increased by raising the extracellular potassium level or the addition of a calcium ionophore, but the time course of stimulated efflux was slow and persistent. These results show that this morphologically undifferentiated cell line is capable of acetylcholine synthesis and expresses markers for synaptic vesicles as well as proteins implicated in calcium-dependent release but lacks an organized release mechanism. |
| Databáze: | OpenAIRE |
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