Engineering and application of polymerases for synthetic genetics
| Autor: | Sebastian Arangundy-Franklin, Philipp Holliger, Gillian Houlihan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Models Molecular Base pair Biomedical Engineering Bioengineering Biology 010402 general chemistry 01 natural sciences Nucleobase 03 medical and health sciences Nucleotide Base Pairing Polymerase Genetics chemistry.chemical_classification Nucleotides Protein engineering 0104 chemical sciences Enzymes Crosstalk (biology) 030104 developmental biology Biochemistry chemistry Nucleic acid biology.protein Alphabet Genetic Engineering Biotechnology |
| Zdroj: | Current opinion in biotechnology. 48 |
| ISSN: | 1879-0429 |
| Popis: | Organic chemistry has systematically probed the chemical determinants of function in nucleic acids by variation to the nucleobase, sugar ring and backbone moieties to build synthetic genetic polymers. Concomitantly, protein engineering has advanced to allow the discovery of polymerases capable of utilizing modified nucleotide analogs. A conjunction of these two lines of investigation in nucleotide chemistry and molecular biology has given rise to a new field of synthetic genetics dedicated to the exploration of the capacity of these novel, synthetic nucleic acids for the storage and propagation of genetic information, for evolution and for crosstalk, that is, information exchange with the natural genetic system. Here we summarize recent progress in synthetic genetics, specifically in the design of novel unnatural basepairs to expand the genetic alphabet as well as progress in engineering polymerases capable of templated synthesis, reverse transcription and evolution of synthetic genetic polymers. |
| Databáze: | OpenAIRE |
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