Synthesis and biological activities of a library of glycosaminoglycans mimetic oligosaccharides
| Autor: | Hemalata Narasimprakash, Denis Barritault, Fernando Siñeriz, Dulce Papy-Garcia, Emmanuel Petit, P. Kern, Arlette Duchesnay, Veronique Barbier-Chassefiere, Said Charef, Mohand-Ouidir Ouidja, Isabelle Martelly, Yasunori Ikeda |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Biophysics Oligosaccharides Bioengineering Cell Line Biomaterials Glycosaminoglycan Mice chemistry.chemical_compound In vivo Skin Ulcer Animals Glycosaminoglycans chemistry.chemical_classification Wound Healing Tissue Engineering Depolymerization Biological activity Heparan sulfate Oligosaccharide In vitro Dextran chemistry Biochemistry Mechanics of Materials Ceramics and Composites Heparitin Sulfate |
| Zdroj: | Biomaterials. 32:769-776 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2010.09.043 |
| Popis: | Biologically active oligosaccharides related to glycosaminoglycans are accumulating increased attention because of their therapeutic potential and for their value in mechanistic studies. Heparan mimetics (HMs) are a family of dextran based polymer known to mimic the properties of glycosaminoglycans, and particularly those of heparan sulfates, as to interact with heparin binding proteins. HMs have shown to stimulate tissue repair in various animal models. Here, we use different methods to depolymerize HMs in order to produce a library of related oligosaccharides and study their biological activities. Since HMs were resistant to endoglycanases activities, depolymerization was achieved by chemical approaches. In vitro biological studies showed that HM oligosaccharides can differentially potentiate FGF-2 mitogenic and antithrombotic activities. In vivo, a selected oligosaccharide (H-dp12) showed to be able to regenerate tissue almost as well as the related polymeric product. The very low anticoagulant activity and high biological activity of low mass oligosaccharides give to these products a new therapeutic potential. |
| Databáze: | OpenAIRE |
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