Intracellular Enzymes Contribution to the Biocatalytic Removal of Pharmaceuticals by Trametes hirsuta
| Autor: | Lounès Haroune, Hubert Cabana, Sabrina Saibi, Jean-Philippe Bellenger |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
010501 environmental sciences Trametes hirsuta Lignin 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Bioremediation Extracellular Environmental Chemistry 0105 earth and related environmental sciences Trametes chemistry.chemical_classification biology Laccase Biosorption Cytochrome P450 General Chemistry biology.organism_classification Biodegradation Environmental 030104 developmental biology Enzyme chemistry Biochemistry biology.protein Intracellular |
| Zdroj: | Environmental Science & Technology. 51:897-904 |
| ISSN: | 1520-5851 0013-936X |
| DOI: | 10.1021/acs.est.6b04409 |
| Popis: | The use of white rot fungi (WRF) for bioremediation of recalcitrant trace organic contaminants (TrOCs) is becoming greatly popular. Biosorption and lignin modifying enzymes (LMEs) are the most often reported mechanisms of action. Intracellular enzymes, such as cytochrome P450 (CYP450), have also been suggested to contribute. However, direct evidence of TrOCs uptake and intracellular transformation is lacking. The aim of this study was to evaluate the relative contribution of biosorption, extracellular LMEs activity, TrOCs uptake, and intracellular CYP450 on the removal of six nonsteroidal anti-inflammatories (NSAIs) by Trametes hirsuta. Results show that for most tested NSAIs, LMEs activity and biosorption failed to explain the observed removal. Most tested TrOCs are quickly taken up and intracellularly transformed. Fine characterization of intracellular transformation using ketoprofen showed that CYP450 is not the sole intracellular enzyme responsible for intracellular transformation. The contribution of CYP450 in further transformation of ketoprofen byproducts is also reported. These results illustrate that TrOCs transformation by WRF is a more complex process than previously reported. Rapid uptake of TrOCs and intracellular transformation through diverse enzymatic systems appears to be important components of WRF efficiency toward TrOCs. |
| Databáze: | OpenAIRE |
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