Converting cancer genes into killer genes
| Autor: | Kenneth W. Kinzler, L T Da Costa, Jin Jen, Timothy A. Chan, Tong-Chuan He, Bert Vogelstein |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Tumor suppressor gene
Cell Survival Mutant Biology medicine.disease_cause Kidney Transfection Models Biological Cell Line Neoplasms Gene expression medicine Humans Point Mutation Gene Multidisciplinary Genetic Therapy Oncogenes Suicide gene Genes p53 beta-Galactosidase Recombinant Proteins Purine-Nucleoside Phosphorylase Cell culture Genes Bacterial Cancer research Mutagenesis Site-Directed Tumor Suppressor Protein p53 Carcinogenesis Plasmids Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 93(9) |
| ISSN: | 0027-8424 |
| Popis: | Over the past decade, it has become clear that tumorigenesis is driven by alterations in genes that control cell growth or cell death. Theoretically, the proteins encoded by these genes provide excellent targets for new therapeutic agents. Here, we describe a gene therapy approach to specifically kill tumor cells expressing such oncoproteins. In outline, the target oncoprotein binds to exogenously introduced gene products, resulting in transcriptional activation of a toxic gene. As an example, we show that this approach can be used to specifically kill cells overexpressing a mutant p53 gene in cell culture. The strategy may be generally applicable to neoplastic diseases in which the underlying patterns of genetic alterations or abnormal gene expression are known. |
| Databáze: | OpenAIRE |
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