Quantitative analysis of aromatase, sulfatase and 17beta-HSD(1) mRNA expression in soft tissue metastases of breast cancer
| Autor: | Natsumi Irahara, Tetsuya Taguchi, Yasuo Miyoshi, Shinzaburo Noguchi, Yasuhiro Tamaki |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Cancer Research medicine.medical_specialty 17-Hydroxysteroid Dehydrogenases Estrogen receptor Breast Neoplasms Soft Tissue Neoplasms Gene Expression Regulation Enzymologic Breast cancer Aromatase Internal medicine Progesterone receptor medicine Steroid sulfatase Humans RNA Messenger Promoter Regions Genetic Aged biology Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Sulfatase Soft tissue Membrane Proteins Middle Aged medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic Endocrinology Oncology Cyclooxygenase 2 biology.protein Tumor necrosis factor alpha Female Steryl-Sulfatase |
| Zdroj: | Cancer letters. 243(1) |
| ISSN: | 0304-3835 |
| Popis: | Expression of the estrogen-synthesizing genes aromatase, steroid sulfatase (STS) and 17beta-hydroxysteroid dehydrogenase type1 (17beta-HSD(1)) has been shown to be up-regulated in primary breast cancer tissue but their expression status in metastatic tumor tissue has yet to be determined. The mRNA expression levels of the three estrogen-synthesizing genes as well as of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6 and cyclooxygenase (COX)-2, all of which have been reported to up-regulate the estrogen-synthesizing genes, were determined by means of a real-time PCR assay in 100 primary breast cancer tissues and 15 soft tissue metastases. In addition, PCR-gel electrophoresis was used to determine the proportion (%) of promoter (l.4, l.3, Pll and l.7) usage of aromatase. Aromatase and STS mRNA levels were significantly (P=0.04 and P=0.03, respectively) higher in soft tissue metastases than in primary tumors, while 17beta-HSD(1) mRNA levels tended (P=0.09) to be higher. The proportions of the promoter usages were very similar for primary tumors and soft tissue metastases, and the mRNA levels of TNF-alpha, IL-6 and COX-2 were not significantly different. Levels of aromatase, STS and 17beta-HSD(1) mRNA are up-regulated in soft tissue metastases compared to those in primary tumors, suggesting that intra-tumoral estrogen synthesis may play a significant role in the growth stimulation of tumor cells in soft tissue metastases as in primary tumors. TNF-alpha, IL-6 and COX-2, on the other hand, are unlikely to be implicated in this up-regulation. |
| Databáze: | OpenAIRE |
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