Antibiotic furazolidone induces CYP1A but not CYP2E1 subfamily in rat liver
| Autor: | Kentaro Q. Sakamoto, Nobuo Sasaki, Mayumi Ishizuka, Tomomi Kamiya, Noriaki Sakai, Shoichi Fujita, Min Heewon, Yoshinori Ikenaka |
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| Rok vydání: | 2008 |
| Předmět: |
Furazolidone
Blotting Western Isozyme Ames test Western blot polycyclic compounds medicine Cytochrome P-450 CYP1A1 Animals Rats Wistar General Veterinary biology medicine.diagnostic_test CYP1A2 Cytochrome P450 CYP2E1 Molecular biology Anti-Bacterial Agents Rats Blot Isoenzymes Biochemistry Gene Expression Regulation Liver biology.protein Electrophoresis Polyacrylamide Gel Female medicine.drug |
| Zdroj: | The Journal of veterinary medical science. 70(3) |
| ISSN: | 0916-7250 |
| Popis: | Furazolidone (FZ), one of the nitrofuran fungicides, is used as a veterinary medicine in the Middle and Far Eastern countries. In this study, FZ (125 mg/kg) was administered orally to Wistar rats for 3 days. Results of the Ames test using the S-9 fraction of rats treated with FZ showed a significant increase in the number of revertant colonies. Western blot analysis of hepatic CYP isozymes induced by FZ, revealed a remarkable induction of CYP1A1 apoprotein, but CYP1A2 and CYP2E1 apoproteins were not altered. In addition, the expression of CYP1A1 mRNA level in rats treated with FZ by RT-PCR was significantly enhanced by FZ treatment. We concluded that FZ is apparently mutagenic and induces transcription of the CYP1A1 isozyme, which metabolically activates numerous promutagens, in hepatocytes. |
| Databáze: | OpenAIRE |
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