Resistance to infection of long-term cryopreserved human aortic valve allografts

Autor: Karin Burgwitz, Christian Kuehn, Omke E. Teebken, Viola Steffen, Georg Marsch
Rok vydání: 2016
Předmět:
Adult
Graft Rejection
Pulmonary and Respiratory Medicine
Aortic valve
Staphylococcus aureus
Prosthesis-Related Infections
Adolescent
medicine.drug_class
Antibiotics
Microbial Sensitivity Tests
030204 cardiovascular system & hematology
medicine.disease_cause
Staphylococcal infections
Risk Assessment
Microbiology
03 medical and health sciences
0302 clinical medicine
Staphylococcus epidermidis
medicine.artery
Ascending aorta
medicine
Humans
Child
Cryopreservation
Colony-forming unit
biology
Pseudomonas aeruginosa
business.industry
Graft Survival
Antibiotic Prophylaxis
Middle Aged
Staphylococcal Infections
Allografts
biology.organism_classification
medicine.disease
Tissue Donors
Anti-Bacterial Agents
Treatment Outcome
medicine.anatomical_structure
030228 respiratory system
Aortic Valve
Surgery
Cardiology and Cardiovascular Medicine
business
Follow-Up Studies
Zdroj: The Journal of Thoracic and Cardiovascular Surgery. 151:1251-1259
ISSN: 0022-5223
DOI: 10.1016/j.jtcvs.2015.11.029
Popis: To analyze the in vitro antimicrobial activity of 3 antibiotic regimens (group A, gentamicin-piperacillin-vancomycin-metronidazole-amphotericin B; group B, gentamicin-piperacillin-flucloxacillin-metronidazole-amphotericin B; and group C, meropenem-vancomycin-tobramycin-colistin-amphotericin B) used in the processing of cryopreserved human ascending aortic tissue and aortic valves against Staphylococcus epidermidis and Staphylococcus aureus. The results were additionally compared with the infection resistance of cryopreserved ascending aortic tissue against Escherichia coli and Pseudomonas aeruginosa.Each of 10 cryopreserved human allografts (CHAs) was divided into 25 pieces (separating aortic wall and valve). Eighteen segments were microbiologically tested, and 7 pieces underwent scanning electron microscopy. A bacterial solution (4 mL; optical density, 0.20 ± 0.02) was used for contamination. After incubation, the optical density of the solution was measured. CHAs underwent sonication to release viable adherent bacteria. The number of attached bacteria was quantified by the colony forming units per square centimeter of CHA surface.Antibiotic regimen groups B and C were more efficient than group A in eradicating gram-positive organisms adherent to the aortic wall (P.001). Group C showed enhanced resistance against E coli compared with group A or B (P.001), whereas group B appeared to be more effective against P aeruginosa (P.001). With reference to each antibiotic regimen, ascending aortic tissue showed significantly less bacterial contamination with staphylococcal bacteria than valve grafts (P ≤ .01).CHAs possess antibacterial activity despite long-term storage over 5 years. Antibiotic combinations applied during CHA processing have a significant influence on their infection resistance. Ascending aortic tissue shows a significantly enhanced bacterial resistance against staphylococcal bacteria compared with aortic valves.
Databáze: OpenAIRE
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