Visualization of Extracellular Matrix Components within Sectioned Salmonella Biofilms on the Surface of Human Gallstones
| Autor: | John S. Gunn, Joanna M. Marshall, Krista M. D. La Perle, Alan D. Flechtner |
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| Rok vydání: | 2014 |
| Předmět: |
Salmonella
Indoles Immunofluorescence lcsh:Medicine Fluorescent Antibody Technique Pathogenesis Gallstones Matrix (biology) medicine.disease_cause Extracellular matrix Cholelithiasis Cholecystitis and Biliary Colic Gram Negative lcsh:Science 0303 health sciences Multidisciplinary Biofilm matrix Enterobacteriaceae Bacterial Pathogens Extracellular Matrix Cell biology Host-Pathogen Interaction Flagella Medicine Immunohistochemical Analysis Biliary Disorders Research Article Histology Immunology Gastroenterology and Hepatology Flagellum Biology Microbiology 03 medical and health sciences Polysaccharides medicine Humans Adhesins Bacterial Microbial Pathogens 030304 developmental biology 030306 microbiology lcsh:R Biofilm Bacteriology biochemical phenomena metabolism and nutrition biology.organism_classification Bacterial adhesin Biofilms Immunologic Techniques lcsh:Q Bacterial Biofilms |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 2, p e89243 (2014) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0089243 |
| Popis: | Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones. Biofilms, by definition, involve the formation of a bacterial community encased within a protective macromolecular matrix. Previous work has demonstrated the composition of the biofilm matrix to be complex and highly variable in response to altered environmental conditions. Although known to play an important role in bacterial persistence in a variety of contexts, the Salmonella biofilm matrix remains largely uncharacterized under physiological conditions. Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol. In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein. Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule. CsgA was not observed in the biofilm matrix and flagellar expression was tightly restricted to the biofilm-cholesterol interface. Images also revealed the presence of preexisting Enterobacteriaceae encased within the structure of the gallstone. These results demonstrate the use and feasibility of this method while highlighting the importance of studying the native architecture of the gallstone biofilm. A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities. |
| Databáze: | OpenAIRE |
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