The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
| Autor: | T. D. K. Brown, I. J. Foulds, M. E. G. Boursnell, Matthew M. Binns |
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| Rok vydání: | 1985 |
| Předmět: |
Genes
Viral Coronaviridae viruses Infectious bronchitis virus medicine.disease_cause Article Virus coronavirus IBV Virology medicine Cloning Molecular oligonucleotide primers Gene Coronavirus Genetics biology Nucleic Acid Hybridization RNA RNA virus DNA biology.organism_classification Oligodeoxyribonucleotides cDNA cloning Avian infectious bronchitis virus Plasmids |
| Zdroj: | Journal of Virological Methods |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(85)90116-8 |
| Popis: | An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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