Sub-chronic exposure to Kalach 360 SL, Glyphosate-based Herbicide, induced bone rarefaction in female Wistar rats

Autor: Tarek Rebai, Asma Mahmoud, Hassane Oudadesse, Riadh Badraoui, Fatma Ayadi, Bertrand Lefeuvre, Latifa Hamdaoui, Manel Naifer
Přispěvatelé: Université de Sfax - University of Sfax, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Hopital Habib Bourguiba - Habib Bourguiba Hospital [Sfax], This work was supported by grants from the Tunisian Ministry of Higher Education and Scientific Researchand it was carried out in the research unit 12ES15 (previously 99/UR/0860) of the Faculty of Medicine, Sfax-Tunisia. The authors are grateful to Pr. Wassim Hariz and Pr. Kamel Maaloul English Language Teachers at the Faculty of Science of Sfax, for having proofread the manuscript., Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2020
Předmět:
Zdroj: Toxicology
Toxicology, Elsevier, 2020, 436, pp.152412. ⟨10.1016/j.tox.2020.152412⟩
Toxicology, 2020, 436, pp.152412. ⟨10.1016/j.tox.2020.152412⟩
ISSN: 0300-483X
DOI: 10.1016/j.tox.2020.152412
Popis: International audience; We investigated the effects of Kalach 360 SL (KL), Glyphosate (G)-based herbicide, on bone tissue in different groups of female Wistar rats. Group 1 (n = 6) received a standard diet and served as a control, groups 2 and 3 (n = 6 each) received 0.07 ml (D1 126 mg/Kg) and 0.175 ml (D2 315 mg/Kg) of KL dissolved in the water for 60 days. The plasma was used to examine the metabolic balance markers (calcium, phosphorus, phosphatase alkaline (PAL), and vitamin D (vit D) and hormonal status (oestrogen and thyroid hormones). As a result, sub-chronic exposure to KL induced a perturbation of bone metabolism (calcium and phosphorus) and hormonal status disturbance. The histological and immunohistochemical study of the thyroid gland revealed a disturbance in morphological structure and thyroid cells function. Moreover, the KL disrupting eff ;ect on thyroid function was investigated by measuring changes in plasma levels of thyroid hormones. Free triiodothyronine (FT3) and thyroxine (FT4) were decreased in female rats breast-fed from rats treated with D and D2 of KL. This eff ;ect was associated with an increase in the plasma level of thyroid-stimulating hormone (TSH). Thus, that KL leads to hypothyroidism. Decrease in levels of oestrogen and thyroid dysfunction led to a disruption in the skeletal bone. The histological study and SEM in bone results allowed us to observe, in rats exposed to KL, the thinning and discontinuity of bone trabecular with a significant decrease in the number of nodes (intertrabecular links).In conclusion, KL sub-chronic exposure caused an aspect of osteoporosis.
Databáze: OpenAIRE
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