Lectin cytochemistry of the lacrimal sac epithelium in experimental dacryocystitis
| Autor: | Makoto Ishikawa, Seiji Maeda, Tohru Abe, Seiki Konno, Shozo Sakuragi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Peanut agglutinin
Male Pathology medicine.medical_specialty Glycoconjugate Epithelium Dacryocystitis Lectins medicine Animals Soybean agglutinin chemistry.chemical_classification biology Lacrimal Apparatus Lectin General Medicine medicine.disease Lacrimal sac Ophthalmology Disease Models Animal medicine.anatomical_structure chemistry Chronic Disease biology.protein Cytochemistry Rabbits Glycoconjugates |
| Zdroj: | Japanese journal of ophthalmology. 43(2) |
| ISSN: | 0021-5155 |
| Popis: | Purpose: To study the glycoconjugates in the lacrimal sac epithelium of Japanese white rabbits with experimentally induced chronic dacryocystitis. Methods: Chronic dacryocystitis was induced by a subcutaneous injection of albumin followed by an injection of Staphylococcus aureus into the lacrimal sac. The histological appearance of the lacrimal sac was studied using the alcian blue-periodic acid-Schiff sequence. In addition, the specific binding to the lacrimal sac epithelium of Ulex europaeus agglutinin 1, Ricinus communis agglutinin 1, peanut agglutinin, and soybean agglutinin was also studied. Results: Staining with alcian blue-periodic acid-Schiff sequence showed hyperplasia of the goblet cells in the inflamed lacrimal sac epithelium. Lectin cytochemistry revealed specific binding of Ulex europaeus agglutinin 1, Ricinus communis agglutinin 1, peanut agglutinin, and soybean agglutinin to the lacrimal sac epithelium. Conclusions: These results indicated that the composition of glycoconjugates in the lacrimal sac epithelium is markedly changed in dacryocystitis. There seems to be a fundamental abnormality in glycoconjugate synthesis in the chronically inflamed lacrimal sac epithelium. |
| Databáze: | OpenAIRE |
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