Screening and Confirmation of 62 Drugs of Abuse and Metabolites in Urine by Ultra-High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry
| Autor: | I-Lin Tsai, Yufeng J. Tseng, Happy Kuy-Lok Tan, Hsiao-Ju Sun, Te-I Weng, Ching-Hua Kuo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Narcotics
Analyte Health Toxicology and Mutagenesis Urine Barbital Toxicology Mass spectrometry Mass Spectrometry Analytical Chemistry Acetic acid chemistry.chemical_compound Limit of Detection medicine Humans Environmental Chemistry Chromatography High Pressure Liquid Immunoassay Detection limit Autoanalysis Chemical Health and Safety Chromatography medicine.diagnostic_test Heroin Dependence Illicit Drugs Chemistry Reproducibility of Results Reference Standards Substance Abuse Detection Indicators and Reagents Algorithms Methadone medicine.drug |
| Zdroj: | Journal of Analytical Toxicology. 37:642-651 |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/bkt083 |
| Popis: | An ultra-high-performance liquid chromatography--quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) method for the screening and confirmation of 62 drugs of abuse and their metabolites in urine was developed in this study. The most commonly abused drugs, including amphetamines, opioids, cocaine, benzodiazepines (BZDs) and barbiturates, and many other new and emerging abused drugs, were selected as the analytes for this study. Urine samples were diluted 5-fold with deionized water before analysis. Using a superficially porous micro-particulate column and an acetic acid-based mobile phase, 54 basic and 8 acidic analytes could be detected within 15 and 12 min in positive and negative ionization modes, respectively. The MS collision energies for the 62 analytes were optimized, and their respective fragmentation patterns were constructed in the in-house library for confirmatory analysis. The coefficients of variation of the intra- and inter-day precision of the analyte responses all were17.39%. All analytes, except barbital, showed matrix effects of 77-121%. The limits of detection of the 62 analytes were between 2.8 and 187.5 ng/mL, which were lower than their respective cut-off concentrations (20-500 ng/mL). Ten urine samples from patients undergoing methadone treatment were analyzed by the developed UHPLC-QTOF-MS method, and the results were compared with the immunoassay method. |
| Databáze: | OpenAIRE |
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