Pseudomonas aeruginosa Planktonic- and Biofilm-Conditioned Media Elicit Discrete Metabolic Responses in Human Macrophages
| Autor: | Brian P. Tripet, Brian J. Eilers, Valérie Copié, Mary Cloud B. Ammons, Isaac R. Miller, Amanda L. Fuchs, Sage M. Schiller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
immunometabolism Cellular homeostasis Article biofilm Quantitative Biology::Cell Behavior 03 medical and health sciences NMR metabolomics 0302 clinical medicine Immune system Metabolomics bacterial-conditioned media lcsh:QH301-705.5 human macrophages Innate immune system Catabolism Chemistry Biofilm General Medicine Metabolism Quantitative Biology::Genomics Cell biology Pseudomonas aeruginosa Quantitative Biology::Quantitative Methods Metabolic pathway 030104 developmental biology lcsh:Biology (General) 030220 oncology & carcinogenesis |
| Zdroj: | Cells Cells, Vol 9, Iss 2260, p 2260 (2020) Volume 9 Issue 10 |
| ISSN: | 2073-4409 |
| Popis: | Macrophages (M&Phi s) are prevalent innate immune cells, present throughout human bodily tissues where they orchestrate innate and adaptive immune responses to maintain cellular homeostasis. M&Phi s have the capacity to display a wide array of functional phenotypes due to different microenvironmental cues, particularly soluble bacterial secretory products. Recent evidence has emerged demonstrating that metabolism supports M&Phi function and plasticity, in addition to energy and biomolecular precursor production. In this study, 1D 1H-NMR-based metabolomics was used to identify the metabolic pathways that are differentially altered following primary human monocyte-derived M&Phi exposure to P. aeruginosa planktonic- and biofilm-conditioned media (PCM and BCM). Metabolic profiling of PCM- and BCM-exposed M&Phi s indicated a significant increase in glycolytic metabolism, purine biosynthesis, and inositol phosphate metabolism. In addition, these metabolic patterns suggested that BCM-exposed M&Phi s exhibit a hyperinflammatory metabolic profile with reduced glycerol metabolism and elevated catabolism of lactate and amino acids, relative to PCM-exposed M&Phi s. Altogether, our study reveals novel findings concerning the metabolic modulation of human M&Phi s after exposure to secretory microbial products and contributes additional knowledge to the field of immunometabolism in M&Phi s. |
| Databáze: | OpenAIRE |
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