Detection of 500-nucleotide DNA by laser desorption mass spectrometry
| Autor: | N. I. Taranenko, David M. Lubman, Steve L. Allman, Chung-Hsuan Chen, Kai Tang, L. Y. Ch'ang |
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| Rok vydání: | 1994 |
| Předmět: |
MALDI imaging
Chromatography Protein mass spectrometry Base Sequence Chemistry Lasers Organic Chemistry Molecular Sequence Data Analytical chemistry Immunoglobulin Variable Region DNA Picolinic acid Mass spectrometry Capillary electrophoresis–mass spectrometry Sample preparation in mass spectrometry Mass Spectrometry Analytical Chemistry Surface-enhanced laser desorption/ionization chemistry.chemical_compound Matrix-assisted laser desorption/ionization Humans Picolinic Acids Spectroscopy |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 8(9) |
| ISSN: | 0951-4198 |
| Popis: | We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing. |
| Databáze: | OpenAIRE |
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